BNP recombinant protein

BNP, 27-134aa, Human, His tag, E Coli

Cat. Num. AAA203219

• Gene Names: NPPB; BNP
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: BNP; 27-134aa; Human; His tag; E Coli; Brain natriuretic peptide (type B natriuretic peptide); NPPB; GC-B; Brain natriuretic peptide (type B natriuretic peptide) Gamma brain natriuretic peptide; Natriuretic peptide precursor B; Natriuretic peptides B; ; BNP recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid In phosphate-buffered saline (pH 7.4), 40%glycerol, 2mM DTT, 0.1mM PMSF, 1mM EDTA
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MHPLGSPGSA SDLETSGLQE QRNHLQGKLS ELQVEQTSLE PLQESPRPTG VWKSREVATE GIRGHRKMVL YTLRAPRSPK MVQGSGCFGR KMDRISSSSG LGCKVLRRH
• Sequence Length: 134

• Applicable Applications for BNP recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Domain: 27-134aa
• Preparation and Storage: Can be stored at +2°C to +8°C for 1 week.; For long term storage, aliquot and store at -20°C or -80°C.; Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for BNP recombinant protein

BNP (Brain natriuretic peptide) is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. BNP is a 32 amino acid polypeptide and is co-secreted along with a 76 amino acid N-terminal fragment which is biologically inactive. This protein has biological actions including natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Recombinant human BNP, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for BNP recombinant protein

Hormones & Cytokines

References

Harada H., et al. (1989) Cell. 58 (4):729-739 ; Matsuyama T., et al. (1993) Cell 75 (1):83-97;

NCBI and Uniprot Product Information

• NCBI GI #: 4505433
• NCBI GeneID: 4879
• NCBI Accession #: NP_002512
• NCBI GenBank Nucleotide #: NM_002521.2
• UniProt Accession #: P16860; Q6FGY0; Q9P2Q7; B0ZBE9
• Molecular Weight: 14 kDa (129 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: natriuretic peptides B preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide B
• NCBI Official Symbol: NPPB
• NCBI Official Synonym Symbols: BNP
• NCBI Protein Information: natriuretic peptides B
• UniProt Protein Name: Natriuretic peptides B
• UniProt Gene Name: NPPB
• UniProt Synonym Gene Names: BNP(1-32); BNP-32

NCBI Description

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

Uniprot Description

Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.