Typical Testing Data/Standard Curve (for reference only)
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Mouse NADPH oxidase 4 ELISA Kit | Nox4 elisa kit
Mouse NADPH oxidase 4 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.6.3.-; Cell cycle regulation; Nucleolus; Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass
Cellular Component: apical plasma membrane; cell junction; endoplasmic reticulum; focal adhesion; integral to membrane; membrane; mitochondrion; NADPH oxidase complex; nucleus; perinuclear region of cytoplasm; plasma membrane; stress fiber
Molecular Function: NAD(P)H oxidase activity; oxidoreductase activity; oxidoreductase activity, acting on NADH or NADPH, with oxygen as acceptor; superoxide-generating NADPH oxidase activity
Biological Process: bone resorption; cardiac muscle cell differentiation; cell aging; cell morphogenesis; gene expression; homocysteine metabolic process; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell migration; positive regulation of stress fiber formation; superoxide metabolic process; superoxide release
Research Articles on Nox4
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Product Notes
The Mouse Nox4 nox4 (Catalog #AAA2882962) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882962 ELISA Kit recognizes Mouse Nox4. It is sometimes possible for the material contained within the vial of "NADPH oxidase 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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