Western Blot (WB)
(Fig1: Western blot analysis of Annexin A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: A431 cell lysateLane 2: Rat uterus tissue lysate)
Annexin A1 Autoantibody (Anti-ANXA1) ELISA Kit | ANXA1 elisa kit
Human Annexin A1 Autoantibody (Anti-ANXA1) ELISA Kit
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Synonyms
Annexin A1 Autoantibody (Anti-ANXA1); Human Annexin A1 Autoantibody (Anti-ANXA1) ELISA Kit; ANXA1 elisa kit
Reactivity
Human, Mouse, Rat
Form/Format
Liquid; 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.01% Proclin-300.
Concentration
1.0mg/ml (varies by lot)
Applicable Applications for ANXA1 elisa kit
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA (EIA)
Conjugate
Non-conjugated
Preparation and Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Fig1: Western blot analysis of Annexin A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: A431 cell lysateLane 2: Rat uterus tissue lysate)
Western Blot (WB)
(Fig1: Western blot analysis of Annexin A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: A431 cell lysateLane 2: Rat uterus tissue lysate)
Related Product Information for ANXA1 elisa kit
Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca2+ and can bind up to eight Ca2+ ions. Displays Ca2+-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca2+-dependent interaction between phagosomes and the actin cytoskeleton.
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Product Notes
The Human, Mouse, Rat ANXA1 (Catalog #AAA109534) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA109534 ELISA Kit recognizes Human, Mouse, Rat ANXA1. AAA Biotech's Annexin A1 Autoantibody (Anti-ANXA1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA (EIA). Researchers should empirically determine the suitability of the ANXA1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A1 Autoantibody (Anti-ANXA1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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