NMT1 recombinant protein

NMT1, 1-496aa, Human, His tag, E Coli

Cat. Num. AAA206008

• Gene Names: NMT1; NMT
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: NMT1; 1-496aa; Human; His tag; E Coli; Glycylpeptide N-tetradecanoyltransferase 1; NMT; Myristoyl-CoA:protein N-myristoyltransferase 1; NMT1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4)
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADESET AVKPPAPPLP QMMEGNGNGH EHCSDCENEE DNSYNRGGLS PANDTGAKKK KKKQKKKKEK GSETDSAQDQ PVKMNSLPAE RIQEIQKAIE LFSVGQGPAK TMEEASKRSY QFWDTQPVPK LGEVVNTHGP VEPDKDNIRQ EPYTLPQGFT WDALDLGDRG VLKELYTLLN ENYVEDDDNM FRFDYSPEFL LWALRPPGWL PQWHCGVRVV SSRKLVGFIS AIPANIHIYD TEKKMVEINF LCVHKKLRSK RVAPVLIREI TRRVHLEGIF QAVYTAGVVL PKPVGTCRYW HRSLNPRKLI EVKFSHLSRN MTMQRTMKLY RLPETPKTAG LRPMETKDIP VVHQLLTRYL KQFHLTPVMS QEEVEHWFYP QENIIDTFVV ENANGEVTDF LSFYTLPSTI MNHPTHKSLK AAYSFYNVHT QTPLLDLMSD ALVLAKMKGF DVFNALDLME NKTFLEKLKF GIGDGNLQYY LYNWKCPSMG AEKVGLVLQ
• Sequence Length: 496

• Applicable Applications for NMT1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NMT1 recombinant protein

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO. Recombinant human NMT1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for NMT1 recombinant protein

Cancer

References

Glover C.J. et al. (1997) J. Biol. Chem. 272:28680-28689.; Van Damme P. et al. (2012) Proc. Natl. Acad. Sci. u.S.A. 109:12449-12454.;

NCBI and Uniprot Product Information

• NCBI GI #: 10835073
• NCBI GeneID: 4836
• NCBI Accession #: NP_066565
• NCBI GenBank Nucleotide #: NM_021079.4
• UniProt Accession #: P30419; Q9UE09; A8K7C1
• Molecular Weight: 59.2 kDa (519 aa)
• NCBI Official Full Name: glycylpeptide N-tetradecanoyltransferase 1
• NCBI Official Synonym Full Names: N-myristoyltransferase 1
• NCBI Official Symbol: NMT1
• NCBI Official Synonym Symbols: NMT
• NCBI Protein Information: glycylpeptide N-tetradecanoyltransferase 1
• UniProt Protein Name: Glycylpeptide N-tetradecanoyltransferase 1
• Protein Family: Phosphoethanolamine N-methyltransferase
• UniProt Gene Name: NMT1
• UniProt Synonym Gene Names: NMT; NMT 1; Type I N-myristoyltransferase

NCBI Description

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008]

Uniprot Description

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.