Human N-myc (and STAT) interactor ELISA Kit | NMI elisa kit

Human N-myc-interactor, NMI ELISA Kit

Cat. Num. AAA935433

• Reactivity: Human
• Synonyms: N-myc (and STAT) interactor; Human N-myc-interactor; NMI ELISA Kit; Human N-myc-interactor (NMI) ELISA kit; N-myc and STAT interactor; N-myc interactor; N-myc-interactor; NMI elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NMI. No significant cross-reactivity or interference between human NMI and analogues was observed.
• Sequence Length: 307

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 23.5 pg/ml-1500 pg/ml
• Sensitivity: < 5.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NMI elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NMI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NMI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NMI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NMI bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 188219599
• NCBI GeneID: 9111
• NCBI Accession #: NP_004679.2
• NCBI GenBank Nucleotide #: NM_004688.2
• UniProt Accession #: Q13287; Q53TI8; Q9BVE5; B5BU69
• Molecular Weight: 35,057 Da
• NCBI Official Full Name: N-myc-interactor
• NCBI Official Synonym Full Names: N-myc (and STAT) interactor
• NCBI Official Symbol: NMI
• NCBI Protein Information: N-myc-interactor; N-myc interactor
• UniProt Protein Name: N-myc-interactor
• Protein Family: N-myc-interactor
• UniProt Gene Name: NMI
• UniProt Synonym Gene Names: Nmi
• UniProt Entry Name: NMI_HUMAN

NCBI Description

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]

Uniprot Description

NMI: May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5. Belongs to the NMI family.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; JAK-STAT cascade; inflammatory response