Human Nucleoside diphosphate kinase A ELISA Kit | NME1 elisa kit

Human Nucleoside diphosphate kinase A ELISA Kit

Cat. Num. AAA764760

• Gene Names: NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• Reactivity: Human
• Synonyms: Nucleoside diphosphate kinase A; Human Nucleoside diphosphate kinase A ELISA Kit; NME1/Nucleoside diphosphate kinase A/NDK A/NDP kinase A/nm23-H1/Tumor metastatic process-associated protein/Granzyme A-activated Dnase/GAAD/Metastasis inhibition factor nm23; NME1 elisa kit

• Reactivity: Human
• Sequence Length: 177

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NME1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NME1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NME1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NME1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NME1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38045913
• NCBI GeneID: 4830
• NCBI Accession #: NP_937818.1
• NCBI GenBank Nucleotide #: NM_198175.1
• Molecular Weight: 30,137 Da
• NCBI Official Full Name: nucleoside diphosphate kinase A isoform a
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 1
• NCBI Official Symbol: NME1
• NCBI Official Synonym Symbols: NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• NCBI Protein Information: nucleoside diphosphate kinase A
• UniProt Protein Name: Nucleoside diphosphate kinase A
• Protein Family: Nucleoside diphosphate kinase
• UniProt Gene Name: NME1
• UniProt Synonym Gene Names: NDPKA; NM23; NDK A; NDP kinase A; GAAD
• UniProt Entry Name: NDKA_HUMAN

NCBI Description

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported.

Protein type: EC 2.7.4.6; Kinase, nucleoside diphosphate; Protein kinase, histidine; Nucleotide Metabolism - purine; Tumor suppressor; Kinase, other; Nucleotide Metabolism - pyrimidine; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: centrosome; cytoplasm; cytosol; intermediate filament; membrane; mitochondrial outer membrane; myelin sheath; nucleus; perinuclear region of cytoplasm

Molecular Function: ATP binding; deoxyribonuclease activity; gamma-tubulin binding; GTP binding; identical protein binding; intermediate filament binding; magnesium ion binding; nucleoside diphosphate kinase activity; protein binding; protein kinase binding; ribosomal small subunit binding; single-stranded DNA binding

Biological Process: CTP biosynthetic process; DNA metabolic process; endocytosis; GTP biosynthetic process; hippocampus development; lactation; negative regulation of cell proliferation; negative regulation of myeloid leukocyte differentiation; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process; positive regulation of DNA binding; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of apoptosis; response to amine stimulus; response to cAMP; response to testosterone stimulus; UTP biosynthetic process

Disease: Neuroblastoma, Susceptibility To