Activin-B Active Active Protein | INHBB active protein

Recombinant Human Activin-B Active

Cat. Num. AAA145165

• Purity: Greater than 97.0% as determined by Analysis by SDS-PAGE.
• Synonyms: Activin-B Active; Recombinant Human Activin-B Active; Activin B Human Active; Activin-B Human Recombinant; Active; Inhibin beta B (activin AB beta polypeptide); Inhibin; beta-2; Activin beta-B chain; MGC157939; Activin B Active; INHBB active protein

• Host: Nicotiana benthamiana plant
• Purity/Purification: Greater than 97.0% as determined by Analysis by SDS-PAGE.
• Form/Format: Lyophilized from 1mg/ml solution in 0.05M Tris-HCl buffer pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: HHHHHHHHHH GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECG
• Sequence Length: 407

• Solubility: It is recommended to reconstitute the lyophilized Activin B in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. EC50 <5ng/ml is required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.
• Preparation and Storage: Lyophilized Activin B although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Activin B should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for INHBB active protein

Description: Activin B human Recombinant produced in Nicotiana benthamiana plant is a beta-B single chain (aa 293-406) containing 123 amino acids (molecular formula C615H910N178O177S12). Activin B is fused to a 10-His-tag at the N-terminal having the total molecular mass of 14kDa and purified by standard chromatographic techniques.

Introduction: Inhibins are dimeric peptide hormones produced by female ovarian granulose cells and male Sertoli cells as well as a variety of other tissues. Inhibins have two isoforms, A and B, with the same alpha subunit but different beta subunits. Inhibin A is a dimer of alpha and beta A subunits, inhibin B is a dimer of alpha and beta B subunits.Inhibins are thought to inhibit the production of follicle-stimulating hormone (FSH) by the pituitary gland. In addition, Inhibins are also thought to play a role in the control of gametogenesis, and embryonic and fetal development.

Product Categories/Family for INHBB active protein

Cytokines and Growth Factors; Growth Factors; Activin

NCBI and Uniprot Product Information

• NCBI GI #: 154813204
• NCBI GeneID: 3625
• NCBI Accession #: NP_002184.2
• NCBI GenBank Nucleotide #: NM_002193.2
• UniProt Accession #: P09529; Q53T31; Q8N1D3
• Molecular Weight: 45,122 Da
• NCBI Official Full Name: inhibin beta B chain preproprotein
• NCBI Official Synonym Full Names: inhibin, beta B
• NCBI Official Symbol: INHBB
• NCBI Protein Information: inhibin beta B chain; Inhibin, beta-2; activin AB beta polypeptide; activin beta-B chain
• UniProt Protein Name: Inhibin beta B chain
• Protein Family: Inhibin
• UniProt Gene Name: INHBB
• UniProt Entry Name: INHBB_HUMAN

NCBI Description

The inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq, Jul 2008]

Uniprot Description

INHBB: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Cytokine; Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 2cen-q13

Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding; host cell surface receptor binding

Biological Process: fat cell differentiation; activin receptor signaling pathway; defense response; negative regulation of hepatocyte growth factor biosynthetic process; cellular response to starvation; negative regulation of follicle-stimulating hormone secretion; negative regulation of insulin secretion; regulation of apoptosis; ovarian follicle development; cellular response to insulin stimulus; response to mechanical stimulus; regulation of MAPKKK cascade; positive regulation of follicle-stimulating hormone secretion; cell differentiation; cell development; oocyte development; growth