Human NF-kappa-B inhibitor beta ELISA Kit | NFKBIB elisa kit

Human NF-kappa-B inhibitor beta ELISA Kit

Cat. Num. AAA766000

• Gene Names: NFKBIB; IKBB; TRIP9
• Reactivity: Human
• Synonyms: NF-kappa-B inhibitor beta; Human NF-kappa-B inhibitor beta ELISA Kit; NFKBIB/TR-interacting protein 9; beta/IkappaBbeta/I-kappa-B-beta/IkB-B/ikB-B/IkB-beta/IKBB/ikB-beta/NF-kappa-B inhibitor beta/NF-kappa-BIB/nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor/Thyroid receptor-interacting protein 9/TR-interacting protein 9/TRIP-9/TRIP9; NFKBIB elisa kit

• Reactivity: Human
• Sequence Length: 356

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NFKBIB elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NFKBIB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NFKBIB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NFKBIB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NFKBIB can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21361135
• NCBI GeneID: 4793
• NCBI Accession #: NP_002494.2
• NCBI GenBank Nucleotide #: NM_002503.4
• Molecular Weight: 35,530 Da
• NCBI Official Full Name: NF-kappa-B inhibitor beta isoform 1
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
• NCBI Official Symbol: NFKBIB
• NCBI Official Synonym Symbols: IKBB; TRIP9
• NCBI Protein Information: NF-kappa-B inhibitor beta
• UniProt Protein Name: NF-kappa-B inhibitor beta
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIB
• UniProt Synonym Gene Names: IKBB; TRIP9; NF-kappa-BIB; IkB-B; IkB-beta; IkappaBbeta; TR-interacting protein 9; TRIP-9
• UniProt Entry Name: IKBB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

IkB-beta: a protein of the NF-kappa-B inhibitor family. Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA- dependent inactivation. Association with inhibitor kappa B- interacting Ras-like proteins 1 and 2 may also explain the slower degradation. Interacts with ligand-binding domain of thyroid hormone receptor-beta, inhibitor kappa B-interacting Ras-like protein 1, inhibitor kappa B-interacting protein Ras2, p65 (RELA) and c-Rel. Expressed in all tissues examined.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; signal transducer activity; transcription coactivator activity

Biological Process: activation of NF-kappaB transcription factor; cytoplasmic sequestering of NF-kappaB; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; positive regulation of interferon type I production; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription, DNA-dependent