SDS-Page
(Recombinant NFKB1 / p50 (1-434) protein gel 10% SDS-PAGE gel stained with Coomassie blue. MW: 73.9 kDa Purity: ?8 )
NFkB1 p50 Recombinant Protein | NFkB1 recombinant protein
Recombinant NFkB1 p50 (1-434) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant NFKB1 / p50 (1-434) protein gel 10% SDS-PAGE gel stained with Coomassie blue. MW: 73.9 kDa Purity: ?8 )
SDS-Page
(Recombinant NFKB1 / p50 (1-434) protein gel 10% SDS-PAGE gel stained with Coomassie blue. MW: 73.9 kDa Purity: ?8 )
Testing Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Testing Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Testing Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Testing Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Background: NFKB1 (Nuclear Factor Kappa B Subunit 1, also known as CVID12, EBP-1, KBF1, NF-kB1, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, NFkB, p105, p50) is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. The precursor NFKB1 is a 105 kDa protein (p105). The 105 kDa protein can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein (p50). The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB complex plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains; they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFkB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IkB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IkB proteins. This releases NFkB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Uniprot Description
NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; apoptosis; negative regulation of cholesterol transport; positive regulation of transcription, DNA-dependent; negative regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; membrane protein intracellular domain proteolysis; MyD88-independent toll-like receptor signaling pathway; negative regulation of interleukin-12 biosynthetic process; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to copper ion; negative regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to oxidative stress; negative regulation of apoptosis
Research Articles on NFkB1
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Product Notes
The NFkB1 nfkb1 (Catalog #AAA389091) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NFkB1 p50, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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