Nuclear Factor I/B Recombinant Protein | NFIB recombinant protein

Recombinant Nuclear Factor I/B (NFIB)

Cat. Num. AAA2030521

• Gene Names: NFIB; CTF; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Nuclear Factor I/B; Recombinant Nuclear Factor I/B (NFIB); NFIB recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMSKDEERAVKDE LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKKHPCCVLSNPD QKGKIRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGERLMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEQDSG QSGSPSHNDPAKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQ
• Sequence Length: 420

• Applicable Applications for NFIB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 57.0kDa
• Accurate Molecular Mass (KD): 57kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Gln233
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 12654879
• NCBI GeneID: 4781
• Molecular Weight: 33,525 Da
• NCBI Official Full Name: Nuclear factor I/B
• NCBI Official Synonym Full Names: nuclear factor I/B
• NCBI Official Symbol: NFIB
• NCBI Official Synonym Symbols: CTF; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
• NCBI Protein Information: nuclear factor 1 B-type
• UniProt Protein Name: Nuclear factor 1 B-type
• Protein Family: Nuclear factor
• UniProt Gene Name: NFIB
• UniProt Synonym Gene Names: NF1-B; Nuclear factor 1/B; CTF; NF-I/B; NFI-B
• UniProt Entry Name: NFIB_HUMAN

Uniprot Description

NFI-B: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: DNA binding; double-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; pontine nucleus development; glial cell differentiation; anterior commissure morphogenesis; chondrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; DNA replication; negative regulation of DNA binding; hindbrain development