Rabbit anti-Human TRPV5 Polyclonal Antibody | anti-TRPV5 antibody

TRPV5, CT (TRPV5, ECAC1, Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, Epithelial calcium channel 1, Osm-9-like TRP channel 3) (AP)

Cat. Num. AAA6358913

• Gene Names: TRPV5; CAT2; ECAC1; OTRPC3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRPV5; Polyclonal Antibody; CT (TRPV5; ECAC1; Transient receptor potential cation channel subfamily V member 5; Calcium transport protein 2; Epithelial calcium channel 1; Osm-9-like TRP channel 3) (AP); Osm-9-like TRP channel 3; anti-TRPV5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TRPV5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TRPV5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 633-663 amino acids from the C-terminal region of human TRPV5.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TRPV5 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TRPV5 antibody detected the TRPV5 protein (arrow).)

Related Product Information for anti-TRPV5 antibody

This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level.

Product Categories/Family for anti-TRPV5 antibody

Antibodies; Transport Proteins

References

Chamoux, E., et al. J. Biol. Chem. 285(33):25354-25362(2010). Kennedy, B.G., et al. Mol. Vis. 16, 665-675 (2010): Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). de Groot, T., et al. J. Am. Soc. Nephrol. 20(8):1693-1704(2009). Semenova, S.B., et al. Am. J. Physiol., Cell Physiol. 296 (5), C1098-C1104 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 17505200
• NCBI GeneID: 56302
• NCBI Accession #: NP_062815.2
• NCBI GenBank Nucleotide #: NM_019841.4
• UniProt Accession #: Q9NQA5; Q8NDW5; Q8NDX7; Q8NDX8; Q96PM6; A4D2H7
• Molecular Weight: 90kDa
• NCBI Official Full Name: transient receptor potential cation channel subfamily V member 5
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily V, member 5
• NCBI Official Symbol: TRPV5
• NCBI Official Synonym Symbols: CAT2; ECAC1; OTRPC3
• NCBI Protein Information: transient receptor potential cation channel subfamily V member 5; ECaC; calcium transporter 2; osm-9-like TRP channel 3; calcium transport protein 2; epithelial calcium channel 1
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 5
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPV5
• UniProt Synonym Gene Names: ECAC11 PublicationManual assertion based on opinion iniRef.1; ECaC; ECaC1; OTRPC3

Uniprot Description

Constitutively active calcium selective cation channel thought to be involved in Ca2+ reabsorption in kidney and intestine (PubMed:11549322, PubMed:18768590). Required for normal Ca2+ reabsorption in the kidney distal convoluted tubules (). The channel is activated by low internal calcium level and the current exhibits an inward rectification (PubMed:11549322, PubMed:18768590). A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay (). Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating ().