WHSC2 recombinant protein

WHSC2, 1-539aa, Human, His tag, E Coli

Cat. Num. AAA203792

• Gene Names: NELFA; WHSC2; NELF-A; P/OKcl.15
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: WHSC2; 1-539aa; Human; His tag; E Coli; Wolf-Hirschhorn syndrome candidate 2; NELF-A; NELFA; P/OKcl.15; Negative elongation factor A; WHSC2 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 20% glycerol
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPGQRRALSP KMASMRESDT GLWLHNKLGA TDELWAPPSI ASLLTAAVID NIRLCFHGLS SAVKLKLLLG TLHLPRRTVD EMKGALMEII QLASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP VKHFQLKRKP KSATLRAELL QKSTETAQQL KRSAGVPFHA KGRGLLRKMD TTTPLKGIPK QAPFRSPTAP SVFSPTGNRT PIPPSRTLLR KERGVKLLDI SELDMVGAGR EAKRRRKTLD AEVVEKPAKE ETVVENATPD YAAGLVSTQK LGSLNNEPAL PSTSYLPSTP SVVPASSYIP SSETPPAPSS REASRPPEEP SAPSPTLPAQ FKQRAPMYNS GLSPATPTPA APTSPLTPTT PPAVAPTTQT PPVAMVAPQT QAPAQQQPKK NLSLTREQMF AAQEMFKTAN KVTRPEKALI LGFMAGSREN PCQEQGDVIQ IKLSEHTEDL PKADGQGSTT MLVDTVFEMN YATGQWTRFK KYKPMTNVS
• Sequence Length: 539

• Applicable Applications for WHSC2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for WHSC2 recombinant protein

WHSC2, also known as NELF-A, is a protein factor required for DRB-sensitive transcription. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Wolf-Hirschhorn syndrome is a multiple malformation syndrome characterized by mental and developmental defects resulting from a hemizygous deletion of the distal short arm of chromosome 4 (4p16.3). Recombinant human WHSC2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for WHSC2 recombinant protein

Transcription & Translation

References

Care A., et al. (2007) Nat Med. 13 (5):613-8.; Mariotti M., et al. (2000) FEBS Lett. 487 (2):166-70.;

NCBI and Uniprot Product Information

• NCBI GI #: 194733742
• NCBI GeneID: 7469
• NCBI Accession #: NP_005654
• NCBI GenBank Nucleotide #: NM_005663.4
• UniProt Accession #: Q9H3P2; O95392; A2A2T1
• Molecular Weight: 60.6 kDa (559aa)
• NCBI Official Full Name: negative elongation factor A
• NCBI Official Synonym Full Names: negative elongation factor complex member A
• NCBI Official Symbol: NELFA
• NCBI Official Synonym Symbols: WHSC2; NELF-A; P/OKcl.15
• NCBI Protein Information: negative elongation factor A
• UniProt Protein Name: Negative elongation factor A
• UniProt Gene Name: NELFA
• UniProt Synonym Gene Names: WHSC2; NELF-A

NCBI Description

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure-dependent manner. Probably required to interact with the RNA polymerase II complex.