Wolf-Hirschhorn Syndrome Candidate 2 Protein Recombinant Protein | NELFA recombinant protein

Wolf-Hirschhorn Syndrome Candidate 2 Protein (WHSC2) Recombinant, Human

Cat. Num. AAA636021

• Gene Names: NELFA; WHSC2; NELF-A; P/OKcl.15
• Applications: ELISA
• Purity: Highly Purified; ~90%
• Synonyms: Wolf-Hirschhorn Syndrome Candidate 2 Protein; Wolf-Hirschhorn Syndrome Candidate 2 Protein (WHSC2) Recombinant; Human; NELFA recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; ~90%
• Form/Format: Supplied as a liquid in 10mM Tris, pH 8.0, 0.1% Triton-X100, 10mM DTT, 0.002% sodium azide.

• Applicable Applications for NELFA recombinant protein: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA as an Antigen, Ligand, or Assay Standard. Tested in MassSspectrometry.
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

SDS-Page

(SDS: Analysis of WHSC2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining)

Related Product Information for NELFA recombinant protein

The gene encoding this protein is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. The gene encoding this protein is mapped to the 165kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. This protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.

Recombinant full-length (525aa) human Wolf-Hirschhorn Syndrome Candidate 2 Protein variant NELFA (NP_005654.1) with T7 tag at the N terminus

Product Categories/Family for NELFA recombinant protein

Molecular Biology; MB-Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 3860187
• NCBI GeneID: 7469
• UniProt Accession #: Q9H3P2; O95392; A2A2T1
• Molecular Weight: 57.35kD
• NCBI Official Full Name: Wolf-Hirschhorn syndrome candidate 2 protein
• NCBI Official Synonym Full Names: negative elongation factor complex member A
• NCBI Official Symbol: NELFA
• NCBI Official Synonym Symbols: WHSC2; NELF-A; P/OKcl.15
• NCBI Protein Information: negative elongation factor A; wolf-Hirschhorn syndrome candidate 2 protein
• UniProt Protein Name: Negative elongation factor A
• UniProt Gene Name: NELFA
• UniProt Synonym Gene Names: WHSC2; NELF-A
• UniProt Entry Name: NELFA_HUMAN

NCBI Description

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]

Uniprot Description

WHSC2: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. Belongs to the NELF-A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; multicellular organismal development; RNA elongation from RNA polymerase II promoter; gene expression