NEK6 recombinant protein

NEK6 protein

Cat. Num. AAA5304419

• Gene Names: NEK6; SID6-1512
• Purity: NEK6 protein was purified using a a proprietary chromatographic technique.
• Synonyms: NEK6; NEK6 protein; NEK-6; NEK 6; Serine/threonine-protein kinase Nek6 protein; NEK 6 protein; NEK protein; NEK-6 protein; NEK6 recombinant protein

• Host: Human
• Purity/Purification: NEK6 protein was purified using a a proprietary chromatographic technique.
• Form/Format: Supplied in a storage buffer consisting of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol
• Sequence Length: 306

• Protein Type: Recombinant
• Biological Significance: Nek6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA family. Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis suggesting that Nek6 is required for metaphase-anaphase transition. Nercc1/Nek9 binds to Nek6 and is likely to be responsible for the activation of Nek6 during mitosis representing a new signaling pathway that regulates mitotic progression.
• Expression System: Sf-9 insect cells
• Preparation and Storage: Store at -80 degree C

Related Product Information for NEK6 recombinant protein

Purified recombinant Human NEK6 protein

Product Categories/Family for NEK6 recombinant protein

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 49457438
• NCBI GeneID: 10783
• UniProt Accession #: Q9HC98; Q5TBG3; Q5TBG9; Q6FG86; Q6IAR3; Q96E83; Q9ULX2; B7Z2D9
• Molecular Weight: 63 kDa
• NCBI Official Full Name: NEK6, partial
• NCBI Official Synonym Full Names: NIMA-related kinase 6
• NCBI Official Symbol: NEK6
• NCBI Official Synonym Symbols: SID6-1512
• NCBI Protein Information: serine/threonine-protein kinase Nek6
• UniProt Protein Name: Serine/threonine-protein kinase Nek6
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: NEK6
• UniProt Synonym Gene Names: NimA-related protein kinase 6
• UniProt Entry Name: NEK6_HUMAN

NCBI Description

The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

NEK6: a protein kinase of the NEK family. Important in mitosis. May regulate spindle function.

Protein type: Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family

Chromosomal Location of Human Ortholog: 9q33.3-q34.11

Cellular Component: spindle pole; microtubule; cytoplasm; microtubule organizing center; nuclear speck; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; signal transducer activity; protein binding; kinesin binding; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: mitosis; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; protein amino acid autophosphorylation; regulation of mitotic metaphase/anaphase transition; regulation of mitotic cell cycle; mitotic nuclear envelope disassembly; cytokinesis; spindle assembly; mitotic cell cycle; G2/M transition DNA damage checkpoint; signal transduction; protein amino acid phosphorylation; chromosome segregation