Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
NEK3 recombinant protein
NEK3, Active
Applications
Western Blot
Synonyms
NEK3; Active; HSPK36; MGC29949; NEK3 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol
Applicable Applications for NEK3 recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for NEK3 recombinant protein
Recombinant full-length human NEK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_002498.
NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a). NEK3 mRNA is detected in all the proliferating cell lines with the amount not changing during the cell cycle (1). During Prolactin receptor signaling, VAV2 is phosphorylated and activated by NEK3. Overexpression of NEK3 in Chinese hamster ovary cells increases cytoskeletal re-organization in response to Prolactin while downregulation of NEK3 expression by siRNA block these effects (2). Prolactin also stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens.
NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a). NEK3 mRNA is detected in all the proliferating cell lines with the amount not changing during the cell cycle (1). During Prolactin receptor signaling, VAV2 is phosphorylated and activated by NEK3. Overexpression of NEK3 in Chinese hamster ovary cells increases cytoskeletal re-organization in response to Prolactin while downregulation of NEK3 expression by siRNA block these effects (2). Prolactin also stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens.
Product Categories/Family for NEK3 recombinant protein
References
1.Kimura, M. et al: Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3). Cytogenet Cell Genet. 2001;95(3-4):177-82.
2.Miller, S.L. et al: Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Oncogene. 2007 Jul 12;26(32):4668-78.
2.Miller, S.L. et al: Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Oncogene. 2007 Jul 12;26(32):4668-78.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~86kDa
Protein Family
Similar Products
Product Notes
The NEK3 (Catalog #AAA517305) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NEK3 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the NEK3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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