Endonuclease 8-like 3 (NEIL3) Recombinant Protein | NEIL3 recombinant protein

Recombinant Human Endonuclease 8-like 3 (NEIL3)

Cat. Num. AAA1313224

• Gene Names: NEIL3; FGP2; FPG2; NEI3; ZGRF3; hFPG2; hNEI3
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Endonuclease 8-like 3 (NEIL3); Recombinant Human Endonuclease 8-like 3 (NEIL3); NEIL3 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-605, Full length protein
• Sequence: VEGPGCTLNGEKIRARVLPGQAVTGVRGSALRSLQGRALRLAASTVVVSPQAAALNNDSSQNVLSLFNGYVYSGVETLGKELFMYFGPKALRIHFGMKGFIMINPLEYKYKNGASPVLEVQLTKDLICFFDSSVELRNSMESQQRIRMMKELDVCSPEFSFLRAESEVKKQKGRMLGDVLMDQNVLPGVGNIIKNEALFDSGLHPAVKVCQLTDEQIHHLMKMIRDFSILFYRCRKAGLALSKHYKVYKRPNCGQCHCRITVCRFGDNNRMTYFCPHCQKENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVLKSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSPPASVCLNDIQHPSKKTTNDITQPSSKVNISPTISSESKLFSPAHKKPKTAQYSSPELKSCNPGYSNSELQINMTDGPRTLNPDSPRCSKHNRLCILRVVGKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRSTMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC
• Sequence Length: 604

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 157388969
• NCBI GeneID: 55247
• NCBI Accession #: NP_060718.2
• NCBI GenBank Nucleotide #: NM_018248.2
• UniProt Accession #: Q8TAT5; Q2PPJ3; Q8NG51; Q9NV95
• Molecular Weight: 67,769 Da
• NCBI Official Full Name: endonuclease 8-like 3
• NCBI Official Synonym Full Names: nei like DNA glycosylase 3
• NCBI Official Symbol: NEIL3
• NCBI Official Synonym Symbols: FGP2; FPG2; NEI3; ZGRF3; hFPG2; hNEI3
• NCBI Protein Information: endonuclease 8-like 3
• UniProt Protein Name: Endonuclease 8-like 3
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL3

NCBI Description

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]

Uniprot Description

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.