Nei Endonuclease VIII-Like 2 Recombinant Protein | NEIL2 recombinant protein

Recombinant Human Nei Endonuclease VIII-Like 2

Cat. Num. AAA144620

• Gene Names: NEIL2; NEH2; NEI2
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Nei Endonuclease VIII-Like 2; Recombinant Human Nei Endonuclease VIII-Like 2; NEIL2 Human; Nei Endonuclease VIII-Like 2 Human Recombinant; Endonuclease 8-like 2; DNA glycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2; Endonuclease VIII-like 2; Nei homolog 2; NEH2; Nei-like protein 2; NEIL2; NEIL2 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: The NEIL2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.; Sterile filtered colorless solution.
• Sequence Positions: 1-332
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMPEGPL VRKFHHLVSP FVGQQVVKTG GSSKKLQPAS LQSLWLQDTQ VHGKKLFLRF DLDEEMGPPG SSPTPEPPQK EVQKEGAADP KQVGEPSGQK TLDGSSRSAE LVPQGEDDSE YLERDAPAGD AGRWLRVSFG LFGSVWVNDF SRAKKANKRG DWRDPSPRLV LHFGGGGFLA FYNCQLSWSS SPVVTPTCDI LSEKFHRGQA LEALGQAQPV CYTLLDQRYF SGLGNIIKNE ALYRAGIHPL SLGSVLSASR REVLVDHVVE FSTAWLQGKF QGRPQHTQVY QKEQCPAGHQ VMKEAFGPED GLQRLTWWCP QCQPQLSEEP EQCQFS.
• Sequence Length: 332

• Tag: 24aa His-tag at the N-terminal
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for NEIL2 recombinant protein

Description: NEIL2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 356 amino acids (1-332) and having a molecular mass of 39.4kDa.NEIL2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Endonuclease 8-like 2 (NEIL2) is a member of a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases set off the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break through the associated lyase reaction. NEIL2 is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL2 has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double stranded DNA (DNA bubbles). NEIL2 has insignificant or undetectable activity with 8-oxoguanine, thymine glycol, 2-hydroxyadenine, hypoxanthine, and xanthine. NEIL2 also has AP (apurinic/apyrimidinic) lyase activity and creates incisions in the DNA strand. NEIL2 cleaves the DNA backbone by beta-delta exclusion to produce a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL2 is found in the testis, skeletal muscle, heart, brain, placenta, lung, pancreas, kidney and liver.

Product Categories/Family for NEIL2 recombinant protein

Enzymes; Endonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 209364526
• NCBI GeneID: 252969
• NCBI Accession #: NP_001129218.1
• NCBI GenBank Nucleotide #: NM_001135746.1
• UniProt Accession #: Q969S2; Q7Z3Q7; Q8N842; Q8NG52; B4DFR7
• Molecular Weight: 39.4 KDa
• NCBI Official Full Name: endonuclease 8-like 2 isoform a
• NCBI Official Synonym Full Names: nei endonuclease VIII-like 2 (E. coli)
• NCBI Official Symbol: NEIL2
• NCBI Official Synonym Symbols: NEH2; NEI2
• NCBI Protein Information: endonuclease 8-like 2; DNA glycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2; endonuclease VIII-like 2; nei homolog 2; nei like 2; nei-like protein 2
• UniProt Protein Name: Endonuclease 8-like 2
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL2
• UniProt Synonym Gene Names: NEH2
• UniProt Entry Name: NEIL2_HUMAN

NCBI Description

NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226])[supplied by OMIM, Mar 2008]

Uniprot Description

NEIL2: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Belongs to the FPG family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Hydrolase; DNA repair, damage; Microtubule-binding; EC 4.2.99.18

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: spindle microtubule; cytoplasm; nucleus

Molecular Function: hydrolase activity, hydrolyzing N-glycosyl compounds; DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; microtubule binding; damaged DNA binding

Biological Process: nucleotide-excision repair; base-excision repair; DNA catabolic process, endonucleolytic