NEDD8 recombinant protein

NEDD8 (human), (recombinant) (His-tag)

Cat. Num. AAA566729

• Gene Names: NEDD8; NEDD-8
• Purity: >=95% (SDS-PAGE)
• Synonyms: NEDD8; NEDD8 (human); (recombinant) (His-tag); NEDD8 recombinant protein

• Host: E Coli
• Purity/Purification: >=95% (SDS-PAGE)
• Form/Format: Liquid. In 20mM HEPES, pH8.0, 50mM NaCl, 1mM DTT

• Source Note: Human NEDD8 is fused with the N-terminal His-tag
• Preparation and Storage: For long term storage, store at -80 degree C; Avoid freeze/thaw cycles. After initial defrost, aliquot into individual tubs and refreeze at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for NEDD8 recombinant protein

NEDD8 is a ubiquitin-like molecule, sharing ~60% identity with ubiquitin at the amino acid level, that can be covalently conjugated (neddylation) to a limited number of cellular proteins in a fashion similar to that of ubiquitin.  The C-terminus of pro-NEDD8, GGLRQ, is efficiently processed to expose glycine76 in the mature form.  This processing is required for conjugation to target proteins via a range of specific activating and conjugating enzymes (Gong and Yeh, 1999). The E1 activating-enzyme complex is composed of two subunits, APP-BP1 and UBA3, whilst the E2 conjugating-enzyme is the ubiquitin E2 UbcH12. The expression of NEDD8 is essential for growth in most organisms where it has been shown to modify the cullin subunit of cullin-based ubiquitin-protein ligases (E3s).  Neddylation acts to regulate the function of these E3s and organisms with lesions in the neddylation process exhibit severe growth defects (Parry and Estelle, 2004). Previously, it was believed that the cullins were the only substrates for NEDDylation, however, it is now recognised that there are a number of such proteins, including the von Hippel-Lindau (VHL) tumour suppressor gene (Stickle et al., 2004) and the tumour suppressor and transcriptional regulator p53 (Harper, 2004) amongst others.

Product Categories/Family for NEDD8 recombinant protein

Cellular Analysis; Stem Cells; Proteostasis; Protein Synthesis & Folding; Posttranslational Modifications; Ubiquitin & UBL Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 5453760
• NCBI GeneID: 4738
• NCBI Accession #: NP_006147.1
• NCBI GenBank Nucleotide #: NM_006156.2
• UniProt Accession #: Q15843; Q3SXN8; Q6LES6
• Molecular Weight: 9,072 Da
• NCBI Official Full Name: NEDD8
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 8
• NCBI Official Symbol: NEDD8
• NCBI Official Synonym Symbols: NEDD-8
• NCBI Protein Information: NEDD8; neddylin; ubiquitin-like protein Nedd8
• UniProt Protein Name: NEDD8
• Protein Family: NEDD8
• UniProt Gene Name: NEDD8
• UniProt Synonym Gene Names: NEDD-8
• UniProt Entry Name: NEDD8_HUMAN

Uniprot Description

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; anatomical structure morphogenesis; protein localization; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance