Hamster Neutrophil Elastase ELISA Kit | NE elisa kit

Hamster Neutrophil Elastase ELISA Kit

Cat. Num. AAA44865

• Gene Names: ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
• Reactivity: Hamster
• Synonyms: Neutrophil Elastase; Hamster Neutrophil Elastase ELISA Kit; NE elisa kit

• Reactivity: Hamster
• Specificity: No significant cross-reactivity or interference between Hamster NE and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 25 ng/ml - 800 ng/ml.
• Sensitivity: 5.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated NE concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster NE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NE and NE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for NE elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated NE concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster NE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NE and NE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503549
• NCBI GeneID: 1991
• NCBI Accession #: NP_001963.1
• NCBI GenBank Nucleotide #: NM_001972.2
• UniProt Accession #: P08246; P09649; Q6B0D9; Q6LDP5
• Molecular Weight: 28,518 Da
• NCBI Official Full Name: neutrophil elastase preproprotein
• NCBI Official Synonym Full Names: elastase, neutrophil expressed
• NCBI Official Symbol: ELANE
• NCBI Official Synonym Symbols: GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
• NCBI Protein Information: neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
• UniProt Protein Name: Neutrophil elastase
• Protein Family: Neutrophil elastase
• UniProt Gene Name: ELANE
• UniProt Synonym Gene Names: ELA2; HLE
• UniProt Entry Name: ELNE_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]

Uniprot Description

ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: EC 3.4.21.37; Cell surface; Protease; Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell surface; transcriptional repressor complex; cytoplasm; extracellular region; secretory granule

Molecular Function: peptidase activity; heparin binding; protein binding; protease binding; cytokine binding; serine-type endopeptidase activity; endopeptidase activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of immune response; positive regulation of smooth muscle cell proliferation; negative regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 biosynthetic process; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; negative regulation of chemotaxis; proteolysis; phagocytosis; cellular calcium ion homeostasis; extracellular matrix disassembly; positive regulation of MAP kinase activity; collagen catabolic process; negative regulation of inflammatory response; defense response to bacterium; protein catabolic process; response to yeast; leukocyte migration; negative regulation of chemokine biosynthetic process; response to UV; acute inflammatory response to antigenic stimulus

Disease: Cyclic Neutropenia; Neutropenia, Severe Congenital, 1, Autosomal Dominant