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NDuFB9 recombinant protein

NDuFB9, 1-179aa, Human, His tag, E Coli (Denatured)

Gene Names
NDUFB9; B22; LYRM3; CI-B22; UQOR22
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
NDuFB9; 1-179aa; Human; His tag; E Coli (Denatured); NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 9; B22; LYRM3; uQOR22; NDuFB9 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAFLASG PYLTHQQKVL RLYKRALRHL ESWCVQRDKY RYFACLMRAR FEEHKNEKDM AKATQLLKEA EEEFWYRQHP QPYIFPDSPG GTSYERYDCY KVPEWCLDDW HPSEKAMYPD YFAKREQWKK LRRESWEREV KQLQEETPPG GPLTEALPPA RKEGDLPPLW WYIVTRPRER PM
Sequence Length
179
Applicable Applications for NDuFB9 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for NDuFB9 recombinant protein
NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 9, also known as NDuFB9, belongs to the complex I LYR family. Localized to the inner mitochondrial membrane, as well as to the matrix side of the peripheral membrane, NDuFB9 functions as an accessory subunit of the multi-subunit mitochondrial membrane respiratory chain NADH dehydrogenase complex I. Complex I plays an important role in the transfer of electrons from NADH to the respiratory chain, a process that is essential for cellular respiration. Recombinant human NDuFB9 protein, fused to His-tag at N-terminus, was expressed in E Coli.
Product Categories/Family for NDuFB9 recombinant protein
References
Loeffen J L., et al. (1998) Biochem Biophys. 253:415-422.; Smeitink J A., et al. (1998) Hum Mol Genet. 7:1573-1579.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24.2kDa (202aa)
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit B9
NCBI Official Symbol
NDUFB9
NCBI Official Synonym Symbols
B22; LYRM3; CI-B22; UQOR22
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
Protein Family
UniProt Gene Name
NDUFB9
UniProt Synonym Gene Names
LYRM3; UQOR22; CI-B22

NCBI Description

The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Research Articles on NDuFB9

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Product Notes

The NDuFB9 ndufb9 (Catalog #AAA204801) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's NDuFB9 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NDuFB9 ndufb9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAFLASG PYLTHQQKVL RLYKRALRHL ESWCVQRDKY RYFACLMRAR FEEHKNEKDM AKATQLLKEA EEEFWYRQHP QPYIFPDSPG GTSYERYDCY KVPEWCLDDW HPSEKAMYPD YFAKREQWKK LRRESWEREV KQLQEETPPG GPLTEALPPA RKEGDLPPLW WYIVTRPRER PM. It is sometimes possible for the material contained within the vial of "NDuFB9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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