Rabbit anti-Human NDUFB9 Polyclonal Antibody | anti-NDUFB9 antibody

NDUFB9 (E134) Polyclonal Antibody

Cat. Num. AAA3013724

• Gene Names: NDUFB9; B22; LYRM3; CI-B22; UQOR22; MC1DN24
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NDUFB9; Polyclonal Antibody; NDUFB9 (E134) Polyclonal Antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; Complex I-B22; CI-B22; LYR motif-containing protein 3; NADH-ubiquinone oxidoreductase B22 subunit; LYRM3; UQOR22; anti-NDUFB9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NDUFB9 (E134) polyclonal antibody detects endogenous levels of NDUFB9 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 179

• Applicable Applications for anti-NDUFB9 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 102-150 of Human NDUFB9.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NDUFB9 (E134) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:EC9706 whole cell lysate (40ug) Lane4:Panc1 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of NDUFB9 (E134) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-NDUFB9 antibody

NDUFB9 (NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9), also known as LYRM3 or B22, is a 179 amino acid protein that belongs to the complex I LYR family. Localized to the inner mitochondrial membrane, as well as to the matrix side of the peripheral membrane, NDUFB9 functions as an accessory subunit of the multi-subunit mitochondrial membrane respiratory chain NADH dehydrogenase complex I. Complex I plays an important role in the transfer of electrons from NADH to the respiratory chain, a process that is essential for cellular respiration.

NCBI and Uniprot Product Information

• NCBI GI #: 6274550
• NCBI GeneID: 4715
• NCBI Accession #: NP_004996.1
• NCBI GenBank Nucleotide #: NP_004996.1
• UniProt Accession #: Q9Y6M9
• Molecular Weight: ~ 22kDa
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase subunit B9
• NCBI Official Symbol: NDUFB9
• NCBI Official Synonym Symbols: B22; LYRM3; CI-B22; UQOR22; MC1DN24
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFB9
• UniProt Synonym Gene Names: LYRM3; UQOR22; CI-B22
• UniProt Entry Name: NDUB9_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

NDUFB9: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I LYR family.

Protein type: EC 1.6.99.3; Mitochondrial; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; EC 1.6.5.3

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; sensory perception of sound; mitochondrial electron transport, NADH to ubiquinone

Disease: Mitochondrial Complex I Deficiency