Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa ELISA Kit | NDUFA10 elisa kit

Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, NDUFA10 ELISA Kit

Cat. Num. AAA9340017

• Gene Names: NDUFA10; CI-42k; CI-42KD
• Reactivity: Human
• Synonyms: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex; 10; 42kDa; Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10; mitochondrial; NDUFA10 ELISA Kit; mitochondrial (NDUFA10) ELISA kit; CI-42KD; FLJ21923; MGC5103; NADH-ubiquinone oxidoreductase 42 kDa subunit; NDUFA10 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human NDUFA10 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated NDUFA10 concentrations in Human serum, plasma and other body fluids. Using Purified Human NDUFA10 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NDUFA10 and NDUFA10 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NDUFA10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for NDUFA10 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated NDUFA10 concentrations in Human serum, plasma and other body fluids. Using Purified Human NDUFA10 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NDUFA10 and NDUFA10 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NDUFA10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758768
• NCBI GeneID: 4705
• NCBI Accession #: NP_004535.1
• NCBI GenBank Nucleotide #: NM_004544.3
• UniProt Accession #: O95299
• Molecular Weight: 40,751 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
• NCBI Official Symbol: NDUFA10
• NCBI Official Synonym Symbols: CI-42k; CI-42KD
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; complex I 42kDa subunit; NADH-ubiquinone oxidoreductase 42 kDa subunit
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFA10
• UniProt Synonym Gene Names: CI-42kD
• UniProt Entry Name: NDUAA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. [provided by RefSeq, Jul 2008]

Uniprot Description

NDUFA10: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA10 subunit family.

Protein type: Mitochondrial; Oxidoreductase; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: mitochondrial matrix; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: nucleoside kinase activity; NADH dehydrogenase (ubiquinone) activity; ATP binding

Biological Process: response to drug; cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; nucleotide biosynthetic process

Disease: Leigh Syndrome