Typical Testing Data/Standard Curve (for reference only)
Rat Neurocan ELISA Kit | NCAN elisa kit
Rat Neurocan ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: NCAN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NCAN antibody and an NCAN-HRP conjugate. The assay sample and buffer are incubated together with NCAN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NCAN concentration since NCAN from samples and NCAN-HRP conjugate compete for the anti-NCAN antibody binding site. Since the number of sites is limited, as more sites are occupied by NCAN from the sample, fewer sites are left to bind NCAN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NCAN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM, Jul 2002]
Uniprot Description
CSPG3: May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. Belongs to the aggrecan/versican proteoglycan family.
Protein type: Secreted, signal peptide; Secreted; Cell adhesion
Chromosomal Location of Human Ortholog: 19p12
Cellular Component: lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region
Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding
Biological Process: chondroitin sulfate metabolic process; axon guidance; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; central nervous system development; glycosaminoglycan metabolic process; chondroitin sulfate catabolic process; carbohydrate metabolic process; regulation of synapse structural plasticity; pathogenesis; cell adhesion; skeletal development; dermatan sulfate biosynthetic process
Research Articles on NCAN
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Product Notes
The Rat NCAN ncan (Catalog #AAA747787) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA747787 ELISA Kit recognizes Rat NCAN. It is sometimes possible for the material contained within the vial of "Neurocan, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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