Western Blot (WB)
(Western blot analysis of SMG7 expression in Jurkat (A), A431 (B), HeLa (C) whole cell lysates.)
Rabbit anti-Human SMG7 Polyclonal Antibody | anti-SMG7 antibody
Anti-SMG7 Antibody
IH (1/100 - 1/200)
IF/IC (1/100 - 1/500)
IP (1/10 - 1/100)
Upon delivery aliquot and store at -20°C for one year.
Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of SMG7 expression in Jurkat (A), A431 (B), HeLa (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of SMG7 expression in Jurkat (A), A431 (B), HeLa (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of SMG7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of SMG7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of SMG7 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of SMG7 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]
Uniprot Description
SMG7: Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1q25
Cellular Component: intermediate filament cytoskeleton; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; protein phosphatase 2A binding
Biological Process: mRNA export from nucleus; mRNA catabolic process, nonsense-mediated decay; regulation of dephosphorylation; gene expression
Research Articles on SMG7
Similar Products
Product Notes
The SMG7 smg7 (Catalog #AAA820862) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SMG7 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMG7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IH), Immunofluorescence/Immunocytochemistry (IF/IC), Immunoprecipitation (IP). WB (1/500 - 1/1000)IH (1/100 - 1/200)
IF/IC (1/100 - 1/500)
IP (1/10 - 1/100). Researchers should empirically determine the suitability of the SMG7 smg7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMG7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
