Visfatin Recombinant Protein | Nampt recombinant protein

Visfatin, 1- 491aa, Mouse, His tag, E Coli

Cat. Num. AAA203101

• Gene Names: Nampt; Pbef; Pbef1; AI314458; AI480535; Visfatin; NAmPRTase; 1110035O14Rik
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Visfatin; 1- 491aa; Mouse; His tag; E Coli; Pre-B-cell colony-enhancing factor 1; visfatin mouse; mouse visfatin; Nicotinamide phosphoribosyltransferase [Mus musculus]; Nampt recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid in Phosphate-Buffered Saline (pH 7.4)
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 1-491
• Sequence: MGSSHHHHHH SSGLVPRGSH MNAAAEAEFN MGSSHHHHHH SSGLVPRGSH MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKVR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERG WNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGHDLLHT VFKNGKVTKS YSFDEVRKNA QLNIEQDVAP H
• Sequence Length: 491

• Applicable Applications for Nampt recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 2-8°C for 1 week. For long term storage,aliquot and store at -20°C to -80°C . Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for Nampt recombinant protein

Visfatin is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (pre-B cell colony-enhancing factor, PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor. Recombinant mouse visfatin was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for Nampt recombinant protein

Hormones & Cytokines

References

Stephens JM., et al. (2006) Curr Opin Lipidol. 17:128-31.; Hug C., et al. (2005) Science. 307 (5708):366-7.;

NCBI and Uniprot Product Information

• NCBI GI #: 257153454
• NCBI GeneID: 59027
• NCBI Accession #: NP_067499
• NCBI GenBank Nucleotide #: NM_021524.2
• UniProt Accession #: Q99KQ4; Q8C3B5; Q9JKM0
• Molecular Weight: 57 kDa (511 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: nicotinamide phosphoribosyltransferase
• NCBI Official Synonym Full Names: nicotinamide phosphoribosyltransferase
• NCBI Official Symbol: Nampt
• NCBI Official Synonym Symbols: Pbef; Pbef1; AI314458; AI480535; Visfatin; NAmPRTase; 1110035O14Rik
• NCBI Protein Information: nicotinamide phosphoribosyltransferase
• UniProt Protein Name: Nicotinamide phosphoribosyltransferase
• Protein Family: Nicotinamide phosphoribosyltransferase
• UniProt Gene Name: Nampt
• UniProt Synonym Gene Names: Pbef1; NAmPRTase; Nampt; PBEF

Uniprot Description

The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma (). Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.