Baculoviral IAP repeat-containing protein 1 Recombinant Protein | NAIP recombinant protein

Recombinant Human Baculoviral IAP repeat-containing protein 1

Cat. Num. AAA1475860

• Gene Names: NAIP; BIRC1; NLRB1; psiNAIP
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Baculoviral IAP repeat-containing protein 1; Recombinant Human Baculoviral IAP repeat-containing protein 1; Neuronal apoptosis inhibitory protein; NAIP recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 60-345aa; Partial
• Sequence: EAKRLKTFVTYEPYSSWIPQEMAAAGFYFTGVKSGIQCFCCSLILFGAGLTRLPIEDHKRFHPDCGFLLNKDVGNIAKYDIRVKNLKSRLRGGKMRYQEEEARLASFRNWPFYVQGISPCVLSEAGFVFTGKQDTVQCFSCGGCLGNWEEGDDPWKEHAKWFPKCEFLRSKKSSEEITQYIQSYKGFVDITGEHFVNSWVQRELPMASAYCNDSIFAYEELRLDSFKDWPRESAVGVAALAKAGLFYTGIKDIVQCFSCGGCLEKWQEGDDPLDDHTRCFPNCPFL
• Sequence Length: 1,241

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for NAIP recombinant protein

Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that ses to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy. Acts as a sensor component of the NLRC4 inflammasome that specifically recognizes and binds needle protein CprI from pathogenic bacteria C. violaceum. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria such as C. violaceum and L. pneumophila.

Product Categories/Family for NAIP recombinant protein

Cancer

References

The gene for neuronal apoptosis inhibitory protein is partially deleted in individuals with spinal muscular atrophy.Roy N., Mahadevan M.S., McLean M., Shutler G., Yaraghi Z., Farahini R., Baird S., Besner-Johnston A., Lefebvre C., Kang X., Salih M., Aubry H., Tamai K., Guan X., Ioannou P., Crawford T.O., de Jong P.J., Surh L., Ikeda J., Korneluk R.G., Mackenzie A.Cell 80:167-178(1995) Sequence of a 131-kb region of 5q13.1 containing the spinal muscular atrophy candidate genes SMN and NAIP.Chen Q., Baird S.D., Mahadevan M., Besner-Johnston A., Farahani R., Xuan J.-Y., Kang X., Lefebvre C., Ikeda J.-E., Korneluk R.G., MacKenzie A.E.Genomics 48:121-127(1998) The DNA sequence and comparative analysis of human chromosome 5.Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S., Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.Nature 431:268-274(2004) Functional human NAIP promoter transcription regulatory elements for the NAIP and PsiNAIP genes.Xu M., Okada T., Sakai H., Miyamoto N., Yanagisawa Y., MacKenzie A.E., Hadano S., Ikeda J.-E.Biochim. Biophys. Acta 1574:35-50(2002) Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes.Liston P., Roy N., Tamai K., Lefebvre C., Baird S., Cherton-Horvat G., Farahani R., McLean M., Ikeda J., Mackenzie A., Korneluk R.G.Nature 379:349-353(1996) The neuronal apoptosis inhibitory protein is a direct inhibitor of caspases 3 and 7.Maier J.K., Lahoua Z., Gendron N.H., Fetni R., Johnston A., Davoodi J., Rasper D., Roy S., Slack R.S., Nicholson D.W., MacKenzie A.E.J. Neurosci. 22:2035-2043(2002) Integrity of ATP binding site is essential for effective inhibition of the intrinsic apoptosis pathway by NAIP.Karimpour S., Davoodi J., Ghahremani M.H.Biochem. Biophys. Res. Commun. 407:158-162(2011) The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus.Zhao Y., Yang J., Shi J., Gong Y.N., Lu Q., Xu H., Liu L., Shao F.Nature 477:596-600(2011) The human apoptosis inhibitor NAIP induces pyroptosis in macrophages infected with Legionella pneumophila.Katagiri N., Shobuike T., Chang B., Kukita A., Miyamoto H.Microbes Infect. 14:1123-1132(2012) Structures of BIR domains from human NAIP and cIAP2.Herman M.D., Moche M., Flodin S., Welin M., Tresaugues L., Johansson I., Nilsson M., Nordlund P., Nyman T.Acta Crystallogr. F 65:1091-1096(2009)

NCBI and Uniprot Product Information

• NCBI GeneID: 4671
• UniProt Accession #: Q13075; O75857; Q13730; Q59GI6; Q8TDZ4; Q99796; B9EG72; E9PHD1
• Molecular Weight: 48.6 kDa
• NCBI Official Synonym Full Names: NLR family, apoptosis inhibitory protein
• NCBI Official Symbol: NAIP
• NCBI Official Synonym Symbols: BIRC1; NLRB1; psiNAIP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 1
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 1
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: NAIP
• UniProt Synonym Gene Names: BIRC1
• UniProt Entry Name: BIRC1_HUMAN

NCBI Description

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This copy of the gene is full length; additional copies with truncations and internal deletions are also present in this region of chromosome 5q13. It is thought that this gene is a modifier of spinal muscular atrophy caused by mutations in a neighboring gene, SMN1. The protein encoded by this gene contains regions of homology to two baculovirus inhibitor of apoptosis proteins, and it is able to suppress apoptosis induced by various signals. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BIRC1: Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor- neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: basolateral plasma membrane; cytoplasm; neuron projection; perikaryon

Molecular Function: ATP binding; cysteine protease inhibitor activity; metal ion binding; protein binding; ubiquitin-protein ligase activity

Biological Process: inflammatory response; innate immune response; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of neuron apoptosis; nervous system development; protein ubiquitination; regulation of signal transduction; response to amino acid stimulus; response to axon injury