MYL2 recombinant protein

Recombinant Human MYL2 protein

Cat. Num. AAA1562225

• Gene Names: MYL2; MLC2; CMH10
• Purity: >90% as determined by SDS-PAGE
• Synonyms: MYL2; Recombinant Human MYL2 protein; Myosin regulatory light chain 2; ventricular/cardiac muscle isoform; MLC-2; MLC-2v; Cardiac myosin light chain 2; Myosin light chain 2; slow skeletal/ventricular muscle isoform; MLC-2s/v; Ventricular myosin light chain 2; MYL2 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human MYL2 (Ala2-Ile159) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for MYL2 recombinant protein

Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly.

Product Categories/Family for MYL2 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 94981553
• NCBI GeneID: 4633
• NCBI Accession #: NP_000423.2
• NCBI GenBank Nucleotide #: NM_000432.3
• UniProt Accession #: P10916; Q16123
• Molecular Weight: 18,789 Da
• NCBI Official Full Name: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• NCBI Official Synonym Full Names: myosin, light chain 2, regulatory, cardiac, slow
• NCBI Official Symbol: MYL2
• NCBI Official Synonym Symbols: MLC2; CMH10
• NCBI Protein Information: myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, ligh
• UniProt Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• Protein Family: Myosin regulatory
• UniProt Gene Name: MYL2
• UniProt Synonym Gene Names: MLC-2; MLC-2v
• UniProt Entry Name: MLRV_HUMAN

NCBI Description

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: sarcomere; cytoskeleton; myofibril; myosin complex; cytosol; A band; actin cytoskeleton

Molecular Function: actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding

Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; post-embryonic development; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Familial Hypertrophic, 10