Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MBS613297.)
Rabbit anti-Human G3BP-1 Polyclonal Antibody
G3BP-1 (GTPase-Activating Protein-Binding Protein 1, GAP SH3 Domain-Binding Protein 1, ATP-dependent DNA helicase VIII, HDH-VIII)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
G3BP-1; Polyclonal Antibody; G3BP-1 (GTPase-Activating Protein-Binding Protein 1; GAP SH3 Domain-Binding Protein 1; ATP-dependent DNA helicase VIII; HDH-VIII); Anti -G3BP-1 (GTPase-Activating Protein-Binding Protein 1; anti-G3BP-1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of total human G3BP-1 protein.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-G3BP-1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 1:50-1:100
Dilution: Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 1:50-1:100
Immunogen
Synthesized non-phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-SP-P-A).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MBS613297.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MBS613297.)
Western Blot (WB)
(Western Blot analysis of extract from 293 cells using MBS613297, lane 1 and 2) and MBS613297-01 (lane 3 and 4))
Western Blot (WB)
(Western Blot analysis of extract from 293 cells using MBS613297, lane 1 and 2) and MBS613297-01 (lane 3 and 4))
Related Product Information for anti-G3BP-1 antibody
G3BP1 (Ras-GTPase activating protein SH3-domain binding protein 1) directly associates with SH3 domains of GTPase activating proteins (GAPs). It was first discovered by its binding to RasGAP. G3BPs have been shown to be involved in a number of mitogenic signaling pathways. G3BP1 is also known to be over expressed in many human cancers (Kim, 2007) and its expression appears to be inversely correlated with PTEN expression (Kim, 2007). Interestingly, G3BP1, along with G3BP2, has been shown to bind to the C-terminus of p53, playing a crucial role in its activity (Kim, 2007). Furthermore, the G3BPs have two traditional RNA binding motifs.
Product Categories/Family for anti-G3BP-1 antibody
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Product Notes
The G3BP-1 (Catalog #AAA613297) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The G3BP-1 (GTPase-Activating Protein-Binding Protein 1, GAP SH3 Domain-Binding Protein 1, ATP-dependent DNA helicase VIII, HDH-VIII) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's G3BP-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 1:50-1:100. Researchers should empirically determine the suitability of the G3BP-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G3BP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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