Myeloid Differentiation Factor 88 (MyD88) Recombinant Protein | MyD88 recombinant protein

Recombinant Myeloid Differentiation Factor 88 (MyD88)

Cat. Num. AAA2010801

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Myeloid Differentiation Factor 88 (MyD88); Recombinant Myeloid Differentiation Factor 88 (MyD88); MyD88 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- RLSLFLNPR TPVAADWTLL AEEMGFEYLE IRELETRPDP TRSLLDAWQG RSGASVGRLL ELLALLDRED ILKELKSRIE EDCQKYLGKQ QNQESEKPLQ VARVESSVPQ TKELGGITTL DDPLGQTPEL FDAFICYCPN DIEFVQEMIR QLEQTDYRLK LCVSDRDVLP GTCVWSIASE LIEKRCRRMV VVVSDDYLQS KECDFQTKFA LSLSPGVQQK RLIPIKYKAM KKDFP
• Sequence Length: 296

• Applicable Applications for MyD88 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg32~Pro265 (Accession # P22366) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6754772
• NCBI GeneID: 17874
• NCBI Accession #: NP_034981.1
• NCBI GenBank Nucleotide #: NM_010851.2
• UniProt Accession #: P22366; O35916
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: myeloid differentiation primary response protein MyD88
• NCBI Official Synonym Full Names: myeloid differentiation primary response gene 88
• NCBI Official Symbol: Myd88
• NCBI Protein Information: myeloid differentiation primary response protein MyD88
• UniProt Protein Name: Myeloid differentiation primary response protein MyD88
• Protein Family: Myeloid differentiation primary response protein
• UniProt Gene Name: Myd88
• UniProt Entry Name: MYD88_MOUSE

Uniprot Description

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: protein complex; postsynaptic density; cytoplasm; plasma membrane; intracellular; cytosol

Molecular Function: identical protein binding; protein binding; interleukin-1 receptor binding; Toll binding; receptor binding

Biological Process: positive regulation of interleukin-23 production; positive regulation of smooth muscle cell proliferation; positive regulation of JNK cascade; response to lipopolysaccharide; response to molecule of fungal origin; signal transduction; activation of NF-kappaB transcription factor; cell surface receptor linked signal transduction; positive regulation of lymphocyte proliferation; response to peptidoglycan; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; JNK cascade; inflammatory response; interferon type I biosynthetic process; positive regulation of interleukin-17 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; cytokine and chemokine mediated signaling pathway; response to virus; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; Toll signaling pathway; regulation of cell proliferation; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; positive regulation of chemokine biosynthetic process; regulation of inflammatory response; innate immune response; immune response; immunoglobulin mediated immune response