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Typical Testing Data/Standard Curve (for reference only)

Human mucin 15, cell surface associated ELISA Kit | MUC15 elisa kit

Human Mucin-15, MUC15 ELISA Kit

Gene Names
MUC15; PAS3; MUC-15; PASIII
Reactivity
Human
Synonyms
mucin 15; cell surface associated; Human Mucin-15; MUC15 ELISA Kit; Human Mucin-15 (MUC15) ELISA kit; PAS3; PASIII; MUC15 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human MUC15. No significant cross-reactivity or interference between Human MUC15 and analogues was observed.
Sequence Length
361
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
125 pg/ml-8000 pg/ml
Sensitivity
31.25 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MUC15 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,294 Da
NCBI Official Full Name
mucin-15 isoform a
NCBI Official Synonym Full Names
mucin 15, cell surface associated
NCBI Official Symbol
MUC15
NCBI Official Synonym Symbols
PAS3; MUC-15; PASIII
NCBI Protein Information
mucin-15
UniProt Protein Name
Mucin-15
Protein Family
UniProt Gene Name
MUC15
UniProt Synonym Gene Names
UNQ750/PRO1481; MUC-15
UniProt Entry Name
MUC15_HUMAN

Uniprot Description

MUC15: May play a role in the cell adhesion to the extracellular matrix. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 11p14.3

Cellular Component: Golgi lumen; plasma membrane; extracellular region; integral to membrane

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; post-translational protein modification

Research Articles on MUC15

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Product Notes

The Human MUC15 muc15 (Catalog #AAA917220) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA917220 ELISA Kit recognizes Human MUC15. It is sometimes possible for the material contained within the vial of "mucin 15, cell surface associated, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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