Rabbit Cathepsin LVKH Monoclonal Antibody | anti-CTSL1 antibody

Cathepsin LVKH Antibody

Cat. Num. AAA9436202

• Gene Names: CTSL1; MEP; CATL; CTSL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: Cathepsin LVKH; Monoclonal Antibody; Cathepsin LVKH Antibody; anti-CTSL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: JM10-78
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-CTSL1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-5000; IHC: 1:50-1:200; ICC: 1:50-1:100; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: A549)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Cathepsin L/V/K/H in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-CTSL1 antibody

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsin L (also designated major excreted protein, MEP or CATL) is a member of the peptidase C1 family and has been identified as a protein that is most closely related to cathepsin H. It is a lysosomal cysteine proteinase that mediates intracellular protein catabolism for collagen, elastin and -1 protease inhibitor. Cathepsin L is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. Transformed mouse fibroblasts stimulated by growth factors or tumor promoters secrete a form of cathepsin L.

Product Categories/Family for anti-CTSL1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 384081592
• NCBI GeneID: 1514
• NCBI Accession #: NP_001244900.1
• NCBI GenBank Nucleotide #: NM_001257971.1
• UniProt Accession #: P07711; Q6IAV1; Q96QJ0
• Molecular Weight: 37,564 Da
• NCBI Official Full Name: cathepsin L1 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cathepsin L1
• NCBI Official Symbol: CTSL1
• NCBI Official Synonym Symbols: MEP; CATL; CTSL
• NCBI Protein Information: cathepsin L1; major excreted protein
• UniProt Protein Name: Cathepsin L1
• UniProt Gene Name: CTSL1
• UniProt Synonym Gene Names: CTSL; MEP
• UniProt Entry Name: CATL1_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

CTSL1: Important for the overall degradation of proteins in lysosomes. Belongs to the peptidase C1 family.

Protein type: Motility/polarity/chemotaxis; EC 3.4.22.15; Protease

Chromosomal Location of Human Ortholog: 9q21.33

Cellular Component: extracellular space; lysosomal lumen; lysosome; extracellular region; nucleus

Molecular Function: collagen binding; protein binding; proteoglycan binding; histone binding; fibronectin binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: antigen processing and presentation; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; adaptive immune response; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis