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Western Blot (WB) (Western Blot: Sample: Recombinant MT3, Mouse.)

Rabbit anti-Mouse Metallothionein 3 (MT3) Polyclonal Antibody | anti-MT3 antibody

Polyclonal Antibody to Metallothionein 3 (MT3)

Gene Names
Mt3; Mt-3
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Metallothionein 3 (MT3); Polyclonal Antibody; Polyclonal Antibody to Metallothionein 3 (MT3); anti-MT3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MT3 antibody
Western Blot (WB), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Trait
Liquid
Cross Reactivity
Mouse
Immunogen
Recombinant MT3 (Met1~Gln68) expressed in E.coli ( MBS2018780 )
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant MT3, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant MT3, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
metallothionein-3
NCBI Official Synonym Full Names
metallothionein 3
NCBI Official Symbol
Mt3
NCBI Official Synonym Symbols
Mt-3
NCBI Protein Information
metallothionein-3
UniProt Protein Name
Metallothionein-3
Protein Family
UniProt Gene Name
Mt3
UniProt Synonym Gene Names
MT-3; GIF; MT-III

Uniprot Description

MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family.

Chromosomal Location of Human Ortholog: 8 C5|8 46.29 cM

Cellular Component: synaptic vesicle

Molecular Function: metal ion binding; zinc ion binding

Biological Process: astrocyte development; cadmium ion homeostasis; cellular lipid catabolic process; cellular metal ion homeostasis; cellular zinc ion homeostasis; cholesterol catabolic process; energy reserve metabolic process; leptin-mediated signaling pathway; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of neurogenesis; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of catalytic activity; positive regulation of transcription, DNA-templated; regulation of protein amino acid glycosylation; regulation of response to food; response to oxidative stress; zinc II ion transport

Research Articles on MT3

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Product Notes

The MT3 mt3 (Catalog #AAA2013371) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Metallothionein 3 (MT3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Metallothionein 3 (MT3) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MT3 mt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Metallothionein 3 (MT3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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