Typical Testing Data/Standard Curve (for reference only)
Human Metallothionein 3 ELISA Kit | MT 3 elisa kit
Human Metallothionein 3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: MT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MT3 antibody and an MT3-HRP conjugate. The assay sample and buffer are incubated together with MT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MT3 concentration since MT3 from samples and MT3-HRP conjugate compete for the anti-MT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MT3 from the sample, fewer sites are left to bind MT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family.
Chromosomal Location of Human Ortholog: 16q13
Cellular Component: synaptic vesicle; perinuclear region of cytoplasm; cytoplasm; intracellular; inclusion body
Molecular Function: antioxidant activity; protein binding; copper ion binding; zinc ion binding; caspase inhibitor activity; drug binding; protein kinase activator activity; cadmium ion binding
Biological Process: positive regulation of catalytic activity; negative regulation of autophagy; removal of superoxide radicals; positive regulation of transcription, DNA-dependent; negative regulation of caspase activity; negative regulation of axon extension; positive regulation of vascular endothelial growth factor receptor signaling pathway; zinc ion homeostasis; cadmium ion homeostasis; cellular lipid catabolic process; histone modification; protein kinase B signaling cascade; negative regulation of neuron apoptosis; cholesterol catabolic process; regulation of response to food; protein stabilization; activation of protein kinase B; cellular zinc ion homeostasis; zinc ion transport; regulation of protein amino acid glycosylation; astrocyte development; leptin-mediated signaling pathway; cell proliferation; cellular metal ion homeostasis; protein import into nucleus, translocation; negative regulation of oxidoreductase activity; response to hypoxia; energy reserve metabolic process; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Research Articles on MT 3
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Product Notes
The Human MT 3 mt3 (Catalog #AAA733923) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA733923 ELISA Kit recognizes Human MT 3. It is sometimes possible for the material contained within the vial of "Metallothionein 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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