Human Metallothionein 3 ELISA Kit | MT3 elisa kit

Human Metallothionein 3 ELISA Kit

Cat. Num. AAA763035

• Gene Names: MT3; GIF; GIFB; GRIF; ZnMT3
• Reactivity: Human
• Synonyms: Metallothionein 3; Human Metallothionein 3 ELISA Kit; MT3/Metallothionein 3; MT3 elisa kit

• Reactivity: Human
• Sequence Length: 68

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MT3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human MT3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MT3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MT3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MT3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15341818
• NCBI GeneID: 4504
• Molecular Weight: 6,927 Da
• NCBI Official Full Name: Metallothionein 3
• NCBI Official Synonym Full Names: metallothionein 3
• NCBI Official Symbol: MT3
• NCBI Official Synonym Symbols: GIF; GIFB; GRIF; ZnMT3
• NCBI Protein Information: metallothionein-3
• UniProt Protein Name: Metallothionein-3
• Protein Family: Metallothionein
• UniProt Gene Name: MT3
• UniProt Synonym Gene Names: MT-3; GIF; MT-III
• UniProt Entry Name: MT3_HUMAN

Uniprot Description

MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family.

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: axon; cytoplasm; cytosol; dendritic spine; extracellular space; inclusion body; intracellular; microtubule; mitochondrial outer membrane; perinuclear region of cytoplasm; plasma membrane; postsynaptic density; ribosome; rough endoplasmic reticulum; synaptic vesicle

Molecular Function: antioxidant activity; cadmium ion binding; caspase inhibitor activity; copper ion binding; drug binding; protein binding; protein kinase activator activity; zinc ion binding

Biological Process: activation of protein kinase B; astrocyte development; brain development; cadmium ion homeostasis; cell proliferation; cellular lipid catabolic process; cellular metal ion homeostasis; cellular zinc ion homeostasis; cholesterol catabolic process; energy reserve metabolic process; histone modification; leptin-mediated signaling pathway; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of axon extension; negative regulation of caspase activity; negative regulation of cell growth; negative regulation of neuron apoptosis; negative regulation of oxidoreductase activity; negative regulation of transcription, DNA-dependent; positive regulation of catalytic activity; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein import into nucleus, translocation; protein kinase B signaling cascade; protein stabilization; regulation of protein amino acid glycosylation; regulation of response to food; removal of superoxide radicals; response to hypoxia; response to metal ion; zinc ion homeostasis; zinc ion transport