Human PSP94 ELISA Kit | MSMB elisa kit

Human PSP94 Quick ELISA Kit

Cat. Num. AAA1753949

• Gene Names: MSMB; MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94
• Reactivity: Human
• Synonyms: PSP94; Human PSP94 Quick ELISA Kit; PSP94/MSMB|IGBF; MSMB; MSPB; PIP; PN44; PRPS; PSP57; microseminoprotein beta; MSMB elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MSMB

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 62.5pg/ml-4,000pg/ml
• Sensitivity: <10pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.107|0.235|0.303|0.517|0.887|1.483|2.142|2.385
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|165|633|1502|; Standard deviation|12.87|47.48|112.65|; CV(%)|7.8%|7.5%|7.5%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|143|574|1323; Standard deviation|8.72|50.51|127.01; CV(%)|6.1%|8.8%|9.6%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 165|155|167|152|160|3.51|2.2%; Sample 2: 633|650|669|736|672|32.93|4.9%; Sample 3: 1502|1245|1397|1305|1362|61.30|4.5%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E Coli; Immunogen sequence: S21-I114
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human PSP94 with <10pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human MSMB Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MSMB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human MSMB with immunogen: Expression system for standard: E Coli; Immunogen sequence: S21-I114.; ; To measure Human MSMB, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MSMB in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MSMB in the sample.
• Assay Procedure Step 1: 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for MSMB elisa kit

Beta-microseminoprotein, also known as MSMB or PSP94, is a protein that in humans is encoded by the MSMB gene. It is a member of the immunoglobulin binding factor family. This gene is mapped to 10q11. 23. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. The expression of the encoded protein is found to be decreased in prostate cancer. But transcripts of identical size were found in prostate, respiratory(tracheal, bronchial, and lung) tissues, and the mucosa of the gastric antrum. Thus, the protein may have functions other than those related to male reproduction. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues.

Product Categories/Family for MSMB elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 119574070
• NCBI GeneID: 4477
• UniProt Accession #: P08118; P11999; Q13125; Q6IAY9; Q9UC59; B1API6
• Molecular Weight: 12,865 Da
• NCBI Official Full Name: microseminoprotein, beta-, isoform CRA_c
• NCBI Official Synonym Full Names: microseminoprotein, beta-
• NCBI Official Symbol: MSMB
• NCBI Official Synonym Symbols: MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94
• NCBI Protein Information: beta-microseminoprotein; seminal plasma beta-inhibin; immunoglobulin binding factor; prostate secreted seminal plasma protein; prostate secretory protein of 94 amino acids
• UniProt Protein Name: Beta-microseminoprotein
• Protein Family: Beta-microseminoprotein
• UniProt Gene Name: MSMB
• UniProt Synonym Gene Names: PRSP; IGBF; PSP-94; PSP94
• UniProt Entry Name: MSMB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer. Two alternatively spliced transcript variants encoding different isoforms are described for this gene. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MSMB: Defects in MSMB are the cause of susceptibility to prostate cancer hereditary type 13 (HPC13). It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Belongs to the beta-microseminoprotein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nucleus

Disease: Prostate Cancer, Hereditary, 13