Rabbit anti-Human, Mouse NECAB3 Polyclonal Antibody | anti-NECAB3 antibody

NECAB3 Antibody

Cat. Num. AAA9408053

• Gene Names: NECAB3; NIP1; XB51; STIP3; EFCBP3; SYTIP2; APBA2BP; dJ63M2.4; dJ63M2.5
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: NECAB3; Polyclonal Antibody; NECAB3 Antibody; AB2BP; amyloid beta A4 protein-binding family A member 2-binding; APBA2BP; dJ63M2.4; dJ63M2.5; anti-NECAB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total NECAB3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 362

• Applicable Applications for anti-NECAB3 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:3000; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human NECAB3.
• Target Name: NECAB3
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from COLO cells, HepG2 cells and HT-29 cells, using NECAB3 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using NECAB3 antibody.)

Related Product Information for anti-NECAB3 antibody

Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta-amyloid.Sugita S., Neuroscience 112:51-63(2002).Yoo J.C., Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.Ota T., Nat. Genet. 36:40-45(2004).

Product Categories/Family for anti-NECAB3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 149158725
• NCBI GeneID: 63941
• NCBI Accession #: NP_112508.3
• NCBI GenBank Nucleotide #: NM_031231.3
• UniProt Accession #: Q96P71; Q5JWF5; Q5JWF6; Q5JWF7; Q86VV1; Q9H433; Q9H8G8; Q9HBW7; Q9HCQ9; A8K780; E1P5N2
• Molecular Weight: 22,251 Da
• NCBI Official Full Name: N-terminal EF-hand calcium-binding protein 3 isoform 1
• NCBI Official Synonym Full Names: N-terminal EF-hand calcium binding protein 3
• NCBI Official Symbol: NECAB3
• NCBI Official Synonym Symbols: NIP1; XB51; STIP3; EFCBP3; SYTIP2; APBA2BP; dJ63M2.4; dJ63M2.5
• NCBI Protein Information: N-terminal EF-hand calcium-binding protein 3
• UniProt Protein Name: N-terminal EF-hand calcium-binding protein 3
• Protein Family: N-terminal EF-hand calcium-binding protein
• UniProt Gene Name: NECAB3
• UniProt Synonym Gene Names: APBA2BP; NIP1; SYTIP2; XB51
• UniProt Entry Name: NECA3_HUMAN

NCBI Description

The protein encoded by this gene interacts with the amino-terminal domain of the neuron-specific X11-like protein (X11L), inhibits the association of X11L with amyloid precursor protein through a non-competitive mechanism, and abolishes the suppression of beta-amyloid production by X11L. This protein, together with X11L, may play an important role in the regulatory system of amyloid precursor protein metabolism and beta-amyloid generation. The protein is phosphorylated by NIMA-related expressed kinase 2, and localizes to the Golgi apparatus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NECAB3: Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta- amyloid. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: endoplasmic reticulum membrane; cytoplasm; Golgi cis cisterna; nucleus

Molecular Function: protein binding; calcium ion binding

Biological Process: regulation of amyloid precursor protein biosynthetic process; protein metabolic process; protein secretion