DNA mismatch repair protein Msh6 Recombinant Protein | MSH6 recombinant protein

Recombinant Human DNA mismatch repair protein Msh6

Cat. Num. AAA9420474

• Gene Names: MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: DNA mismatch repair protein Msh6; Recombinant Human DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBPMutS-alpha 160 kDa subunit; p160; MSH6 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Partial, 1-400aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

NCBI and Uniprot Product Information

• NCBI GI #: 4504191
• NCBI GeneID: 2956
• NCBI Accession #: NP_000170.1
• NCBI GenBank Nucleotide #: NM_000179.2
• UniProt Accession #: P52701; O43706; O43917; Q8TCX4; Q9BTB5; B4DF41; B4E3I4; F5H2F9
• Molecular Weight: 60.03kD
• NCBI Official Full Name: DNA mismatch repair protein Msh6 isoform 1
• NCBI Official Synonym Full Names: mutS homolog 6
• NCBI Official Symbol: MSH6
• NCBI Official Synonym Symbols: GTBP; HSAP; p160; GTMBP; HNPCC5
• NCBI Protein Information: DNA mismatch repair protein Msh6
• UniProt Protein Name: DNA mismatch repair protein Msh6
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MSH6
• UniProt Synonym Gene Names: p160

NCBI Description

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]

Uniprot Description

MSH6: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Belongs to the DNA mismatch repair MutS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; MutSalpha complex; nucleoplasm

Molecular Function: ADP binding; ATP binding; ATPase activity; DNA-dependent ATPase activity; double-stranded DNA binding; four-way junction DNA binding; guanine/thymine mispair binding; magnesium ion binding; methylated histone residue binding; mismatched DNA binding; MutLalpha complex binding; oxidized purine DNA binding; protein binding; protein homodimerization activity; single guanine insertion binding; single thymine insertion binding

Biological Process: determination of adult life span; DNA damage response, signal transduction resulting in induction of apoptosis; DNA repair; isotype switching; maintenance of DNA repeat elements; meiotic mismatch repair; mismatch repair; negative regulation of DNA recombination; positive regulation of helicase activity; response to UV; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments

Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 5; Endometrial Cancer; Mismatch Repair Cancer Syndrome