Rabbit anti-Human CD62P Polyclonal Antibody | anti-CD62P antibody

CD62P Polyclonal Antibody

Cat. Num. AAA3012429

• Gene Names: SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: CD62P; Polyclonal Antibody; CD62P Polyclonal Antibody; GMP-140; LECAM3; PADGEM; SELP; Antigen CD62; CD62; CD62 antigen-like family member P; GMP 140; GMP140; GMRP; Granule membrane protein 140; Granulocyte membrane protein; GRMP; LECAM 3; Leukocyte endothelial cell adhesion molecule 3; Leukocyte-endothelial cell adhesion molecule 3; LYAM3; P Selectin; P-selectin; Platelet activation dependent granule-external membrane protein; Platelet alpha granule membrane protein; PSEL; Selectin P (granule membrane protein 140kDa; antigen CD62); anti-CD62P antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CD62P polyclonal antibody detects endogenous levels of CD62P protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 830

• Applicable Applications for anti-CD62P antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1,000-1:2,000; IHC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of CD62P on different cells lysates using anti-CD62P antibody at 1/500 dilution. Positive control: Line1: HUVEC Line2: Human placenta)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD62P antibody. Counter stained with hematoxylin.)

Related Product Information for anti-CD62P antibody

Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 183389
• NCBI GeneID: 6403
• UniProt Accession #: P16109
• Molecular Weight: 100kDa
• NCBI Official Full Name: granule membrane protein-140
• NCBI Official Synonym Full Names: selectin P
• NCBI Official Symbol: SELP
• NCBI Official Synonym Symbols: CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• NCBI Protein Information: P-selectin
• UniProt Protein Name: P-selectin
• UniProt Gene Name: SELP
• UniProt Synonym Gene Names: GMRP; GRMP; GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_HUMAN

NCBI Description

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]

Uniprot Description

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; integral to plasma membrane; cytoplasm; plasma membrane; platelet dense granule membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; glycosphingolipid binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; heterophilic cell adhesion; platelet activation; leukocyte adhesion; platelet degranulation; regulation of integrin activation; positive regulation of leukocyte migration; response to lipopolysaccharide; defense response to Gram-negative bacterium; blood coagulation; inflammatory response; leukocyte tethering or rolling; cell adhesion; leukocyte migration

Disease: Ige Responsiveness, Atopic