SDS-Page
MRPL28 recombinant protein
MRPL28, 56-256aa, Human, His tag, E Coli
Gene Names
MRPL28; p15; MAAT1
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
MRPL28; 56-256aa; Human; His tag; E Coli; 39S ribosomal protein L28 mitochondrial; 39S ribosomal protein L28; mitochondrial; MAAT1; p15; MRPL28 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MNGQRERVED VPIPIYFPPE SQRGLWGGEG WILGQIYANN DKLSKRLKKV WKPQLFEREF YSEILDKKFT VTVTMRTLDL IDEAYGLDFY ILKTPKEDLC SKFGMDLKRG MLLRLARQDP QLHPEDPERR AAIYDKYKEF AIPEEEAEWV GLTLEEAIEK QRLLEEKDPV PLFKIYVAEL IQQLQQQALS EPAVVQKRAS GQ
Sequence Length
256
Applicable Applications for MRPL28 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for MRPL28 recombinant protein
MRPL28, also as known as 39S ribosomal protein L28, mitochondrial, is a mitochondrial protein that belongs to the ribosomal protein L28P family. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL28 potentially represents an important therapeutic reagent for HLA-A24 (A24) patients as this antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290, which targets the A24 serotype. This protein is found in a variety of normal tissues including spleen, testis, thymus, liver, kidney, brain, adrenal, lung and retinal tissue. Recombinant human MRPL28 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for MRPL28 recombinant protein
References
Koc EC, et al. (2001) J Biol Chem 276 (47): 43958-69.; Kawakami, Y., et al. (2000) J.Immunother. 23: 17-27.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.8kDa (222aa) confirmed by MALDI-TOF
NCBI Official Full Name
39S ribosomal protein L28, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L28
NCBI Official Symbol
MRPL28
NCBI Official Synonym Symbols
p15; MAAT1
NCBI Protein Information
39S ribosomal protein L28, mitochondrial
UniProt Protein Name
39S ribosomal protein L28, mitochondrial
Protein Family
UniProt Gene Name
MRPL28
UniProt Synonym Gene Names
MAAT1; L28mt; MRP-L28
NCBI Description
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]
Uniprot Description
MiscellaneousPotentially represents an important therapeutic reagent for HLA-A24 patients. This antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290 in the context of HLA-A24.
Research Articles on MRPL28
Similar Products
Product Notes
The MRPL28 mrpl28 (Catalog #AAA204795) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's MRPL28 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the MRPL28 mrpl28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MNGQRERVED VPIPIYFPPE SQRGLWGGEG WILGQIYANN DKLSKRLKKV WKPQLFEREF YSEILDKKFT VTVTMRTLDL IDEAYGLDFY ILKTPKEDLC SKFGMDLKRG MLLRLARQDP QLHPEDPERR AAIYDKYKEF AIPEEEAEWV GLTLEEAIEK QRLLEEKDPV PLFKIYVAEL IQQLQQQALS EPAVVQKRAS GQ. It is sometimes possible for the material contained within the vial of "MRPL28, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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