Angiotensin Converting Enzyme 2 Enzyme | ACE2 enzyme

Angiotensin Converting Enzyme 2, Mouse (ACE-2, ACEH) (Control Peptide)

Cat. Num. AAA653645

• Applications: ELISA
• Purity: Highly Purified; Highly Purified
• Synonyms: Angiotensin Converting Enzyme 2; Mouse (ACE-2; ACEH) (Control Peptide); ACE2 enzyme

• Host: Mouse, synthetic peptide
• Specificity: Synthetic peptide 20aa mapped to the internal (ectodomain) of Mouse ACE-2. Species Homology: Human - 70%.
• Purity/Purification: Highly Purified; Highly Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

• Applicable Applications for ACE2 enzyme: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot.; Dilution: ELISA: Coat plates at 1ug/ml; Antibody Blocking: 5-10ug Control peptide per 1ug A2295-01H (affinity purified antibody).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ACE2 enzyme

Control Peptide for A2295-01H (affinity purified antibody).

Renin-Angiotensin System (RAS) is a crtical regulator of blood pressure homeostasis. The protease renin cleaves angiotensinogen into inactive decameric peptide angiotensin-I (Ang-I). Angiotensin-converting enzyme (ACE) then cleaves C-terminal dipeptide from Ang-I to form an active octamer angiotensin-II (Ang-II), which can contribute to hypertension by promoting vascular smooth muscle vasocontriction and renal tubule sodium reabsorption. ACE can also cleave many other small peptides including the vasodialating peptide bradykinin into inactive fragment, cleave Alzheimer amyloid beta-peptide (Abeta), retard Abeta aggregation, deposition and fibril formation. ACE mutant mice display spontaneous hypotension, partial male infertility and kidney malformations. ACE is found in somatic (s-ACE) and testicular/germinal (t-ACE) isoforms. The products of renin and ACE catalysis, namely Ang1-10 and Ang1-8 can also be by another peptidase, ACE-2 to Ang1-9 and Ang1-7, respectively. ACE-2 and ACE (s-ACE and t-ACE) are made as transmembrane (TM) proteins. These enzymes also exist as soluble, truncated forms lacking the TM and cytosolic domains.
ACE-2 (also known as ACE-2 and ACE homolog, ACEH) gene has been mapped at human chromosome Xp22. ACE-2 enzymes from human (805aa) and mouse (798aa) are single chain proteins with 40% seq homology to N- and C-terminal domains of ACE. However, in contrast to s-ACE which consists of two catalytic sites, ACE-2 contains only one active site. Unlike s-ACE and t-ACE which are dipeptidyl-carboxypeptidases, ACE-2 acts as a carboxypeptidase, cleaving single residue from Ang-I, generating Ang1-9 and a single residue from Ang-II to generate Ang1-7. ACE-2 can cleave angiotensin-I but not bradykinin and the enzyme activity is not inhibited by the ACE inhibitors. This enzyme is expressed highly in heart, kidney and testis and moderately in colon, small intestine and ovary. ACE-2 is an essential regulator of heart fuction because targeted disruption of this enzyme in mice results in severe cardiac contractility defect, increased angiotensin-II levels and upregulation of hypoxia-induced genes in the heart.

Product Categories/Family for ACE2 enzyme

Molecular Biology; MB-Enzymes; Angiotensin Converting (ACE)

NCBI and Uniprot Product Information

• NCBI GI #: 170066580
• NCBI GeneID: 100144303
• NCBI Official Full Name: angiotensin converting enzyme 2
• NCBI Official Symbol: ACE2
• NCBI Protein Information: angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme