CCNB1 sirna

CCNB1 siRNA (Mouse)

Cat. Num. AAA826897

• Gene Names: Ccnb1; CycB1; Cycb-4; Cycb-5; Ccnb1-rs1; Cycb1-rs1; Ccnb1-rs13
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCNB1; CCNB1 siRNA (Mouse); CCN-2; CCNB1-RS13; CYCB; CYCB1; G2/mitotic-specific cyclin-B1; CCNB1 sirna

• Reactivity: Mouse
• Specificity: CCNB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 430

• Applicable Applications for CCNB1 sirna: RNA Interference (RNAi)
• Source: Synthetic
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CCNB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCNB1 sirna

siRNA to inhibit CCNB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28195398
• NCBI GeneID: 268697
• NCBI Accession #: NP_758505.2
• NCBI GenBank Nucleotide #: NM_172301.3
• UniProt Accession #: P24860
• Molecular Weight: 48,052 Da
• NCBI Official Full Name: G2/mitotic-specific cyclin-B1
• NCBI Official Synonym Full Names: cyclin B1
• NCBI Official Symbol: Ccnb1
• NCBI Official Synonym Symbols: CycB1; Cycb-4; Cycb-5; Ccnb1-rs1; Cycb1-rs1; Ccnb1-rs13
• NCBI Protein Information: G2/mitotic-specific cyclin-B1
• UniProt Protein Name: G2/mitotic-specific cyclin-B1
• Protein Family: G2/mitotic-specific cyclin
• UniProt Gene Name: Ccnb1
• UniProt Synonym Gene Names: Ccn-2; Ccnb1-rs13; Cycb; Cycb1
• UniProt Entry Name: CCNB1_MOUSE

Uniprot Description

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Activator; Cell cycle regulation

Cellular Component: spindle pole; centrosome; cytoskeleton; membrane; cytoplasm; nucleus

Molecular Function: protein binding; protein complex binding; patched binding; histone kinase activity; kinase activity; protein kinase binding

Biological Process: mitosis; positive regulation of histone phosphorylation; in utero embryonic development; regulation of cell cycle; positive regulation of attachment of spindle microtubules to kinetochore; positive regulation of mRNA 3'-end processing; positive regulation of cell cycle; cell cycle; positive regulation of mitotic cell cycle; oocyte maturation; mitotic metaphase plate congression; protein amino acid phosphorylation; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; cell division; protein complex assembly; regulation of cyclin-dependent protein kinase activity; mitotic spindle stabilization; positive regulation of cardiac muscle cell proliferation