Mouse anti-Human Aurora B Antibody | anti-AURKB antibody

Aurora B (Proliferation Marker)

Cat. Num. AAA4381397

• Gene Names: AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Aurora B; Aurora B (Proliferation Marker); AIK2; AIM-1; ARK-2; AurB; AURKB; Aurora-1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora-B; Aurora-related kinase 2; Aurora/IPL1-related kinase 2; IPL1; Protein phosphatase 1 regulatory subunit 48 (PPP1R48); Serine/threonine-protein kinase 12; Serine/threonine-protein kinase aurora-B; STK1; STK12; STK5; anti-AURKB antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1, kappa
• Clone Number: rAURKB/1592
• Specificity: Recognizes a protein of 39kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 344

• Applicable Applications for anti-AURKB antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HeLa cells. Tonsil, Placenta, Hodgkin s Lymphoma or Colon Carcinoma.
• Immunogen: Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)
• Cellular Localization: Nuclear and Cytoplasmic
• Hu-chromosome Location: 17p13.1
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human kidney stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human kidney stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human placenta stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).)

SDS-Page

(SDS-PAGE Analysis Purified Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592). Confirmation of Integrity and Purity of Antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 83776600
• NCBI GeneID: 9212
• NCBI Accession #: NP_004208.2
• NCBI GenBank Nucleotide #: NP_004208.2
• UniProt Accession #: Q96GD4
• Molecular Weight: 39kDa
• NCBI Official Full Name: aurora kinase B isoform 1
• NCBI Official Synonym Full Names: aurora kinase B
• NCBI Official Symbol: AURKB
• NCBI Official Synonym Symbols: AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
• NCBI Protein Information: aurora kinase B
• UniProt Protein Name: Aurora kinase B
• Protein Family: Aurora kinase
• UniProt Gene Name: AURKB
• UniProt Synonym Gene Names: AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; AIM-1; ARK-2
• UniProt Entry Name: AURKB_HUMAN

NCBI Description

This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

AurB: a member of the AUR family of kinases. May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Expressed during S and G2/M phase and expression is upregulated in cancer cells during M phase. Localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis. Colocalizes with gamma tubulin in the mid-body. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Overexpressed in colorectal and other cancer cell lines and thought to cause aneuploidy via histone phosphorylation.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; AUR family

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: spindle microtubule; spindle; spindle midzone; spindle pole centrosome; midbody; nucleus; cytosol; condensed nuclear chromosome, pericentric region; chromocenter

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein serine/threonine/tyrosine kinase activity; histone serine kinase activity; ATP binding

Biological Process: positive regulation of cytokinesis; spindle stabilization; spindle checkpoint; spindle midzone assembly involved in mitosis; protein amino acid autophosphorylation; regulation of chromosome segregation; negative regulation of transcription from RNA polymerase II promoter; attachment of spindle microtubules to kinetochore; protein amino acid phosphorylation; negative regulation of cytokinesis; cell proliferation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; histone modification; abscission; mitotic cell cycle; negative regulation of protein binding; negative regulation of B cell apoptosis; aging