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Mouse Anti-Human IgE Fc Secondary Antibody | IgE secondary antibody

Mouse Anti-Human IgE Fc-FITC

Applications
ELISA, ELISA, Immunoblot, Western Blot
Synonyms
Mouse Anti-Human IgE Fc; Mouse Anti-Human IgE Fc-FITC; IgE secondary antibody
Ordering
For Research Use Only!
Host
Mouse
Isotype
IgG2akappa
Clone Number
HP6029
Specificity
Human IgE Fc
Concentration
0.5 mg/mL (varies by lot)
Applicable Applications for IgE secondary antibody
ELISA (EIA), FLISA, ELISpot (EIA), Immunoblot (IB), Purification
Application Notes
ELISA - Quality tested; FLISA - Quality tested; ELISpot - Reported in literature; Immunoblotting - Reported in literature; Purification - Reported in literature
Buffer Formulation
Supplied in PBS/NaN3
Isotype Control
Mouse IgG2a-FITC
Preparation and Storage
Store at 2-8 degree C
References
1. Agea E, Russano A, Bistoni O, Mannucci R, Nicoletti I, Corazzi L, et al. Human CD1-restricted T cell recognition of lipids from pollens. J Exp Med. 2005;202:295-308. (ELISA)
2. Milovanovic M, Heine G, Zuberbier T, Worm M. Allergen extract-induced interleukin-10 in human memory B cells inhibits immunoglobulin E production. Clin Exp Allergy. 2009;39:671-8. (ELISA)
3. Heine G, Dahten A, Hilt K, Ernst D, Milovanovic M, Hartmann B, et al. Liver X receptors control IgE expression in B cells. J Immunol. 2009;182:5276-82. (ELISA)
4. Milovanovic M, Drozdenko G, Weise C, Babina M, Worm M. Interleukin-17A promotes IgE production in human B cells. J Invest Dermatol. 2010;130:2621-8. (ELISA)
5. Weise C, Hilt K, Milovanovic M, Ernst D, Rühl R, Worm M. Inhibition of IgE production by docosahexaenoic acid is mediated by direct interference with STAT6 and NFkappaB pathway in human B cells. J Nutr Biochem. 2011;22:269-75. (ELISA, ELISPOT)
6. Sabbatini P, Tsuji T, Ferran L, Ritter E, Sedrak C, Tuballes K, et al. Phase I trial of overlapping long peptides from a tumor self-antigen and poly-ICLC shows rapid induction of integrated immune response in ovarian cancer patients. Clin Cancer Res. 2012;18:6497-508. (ELISA)
7. Qian Y, Jeong JS, Abdeladhim M, Valenzuela JG, Aoki V, Hans-Filhio G, et al. IgE anti-LJM11 sand fly salivary antigen may herald the onset of Fogo selvagem in endemic Brazilian regions. J Invest Dermatol. 2015;135:913-5. (ELISA)
8. Bundhoo A, Paveglio S, Rafti E, Dhongade A, Blumberg RS, Matson AP. Evidence that FcRn mediates the transplacental passage of maternal IgE in the form of IgG anti-IgE/IgE immune complexes. Clin Exp Allergy. 2015 Feb 5. doi: 10.1111/cea.12508. [Epub ahead of print]. (ELISA)
9. Martos G, Pineda-Vadillo C, Miralles B, Alonso-Lebrero E, López-Fandiño R, Molina E, et al. Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion. J Agric Food Chem. 2012;60:5215-20. (WB)
10. Benedé S, López-Expósito I, Giménez G, Grishina G, Bardina L, Sampson HA, et al. In vitro digestibility of bovine beta-casein with simulated and human oral and gastrointestinal fluids. Identification and IgE-reactivity of the resultant peptides. Food Chem. 2014;143:514-21. (WB)
11. Kleine-Tebbe J, Hamilton RG, Roebber M, Lichtenstein LM, MacDonald SM. Purification of immunoglobulin E (IgE) antibodies from sera with high IgE titers. J Immunol Methods. 1995;179:153-64. (Purification, ELISA)

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Product Notes

The IgE (Catalog #AAA670693) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mouse Anti-Human IgE Fc can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), FLISA, ELISpot (EIA), Immunoblot (IB), Purification. ELISA - Quality tested; FLISA - Quality tested; ELISpot - Reported in literature; Immunoblotting - Reported in literature; Purification - Reported in literature. Researchers should empirically determine the suitability of the IgE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse Anti-Human IgE Fc, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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