Mouse X-Box Binding Protein 1 (XBP1) RTU ELISA Kit | XBP1 rtu elisa kit

Mouse X-Box Binding Protein 1 (XBP1) ELISA Kit

Cat. Num. AAA4503404

• Gene Names: Xbp1; TREB5; XBP-1; TREB-5; D11Ertd39e
• Reactivity: Mouse
• Synonyms: X-Box Binding Protein 1 (XBP1); Mouse X-Box Binding Protein 1 (XBP1) ELISA Kit; TREB5; XBP2; Tax-responsive element-binding protein 5; XBP1 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 371

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for XBP1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to XBP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to XBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain XBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of XBP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 411147451
• NCBI GeneID: 22433
• NCBI Accession #: NP_001258659.1
• NCBI GenBank Nucleotide #: NM_001271730.1
• UniProt Accession #: O35426; Q8VHM0; Q922G5; Q9ESS3
• Molecular Weight: 39,888 Da
• NCBI Official Full Name: X-box-binding protein 1 isoform XBP1(S)
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: Xbp1
• NCBI Official Synonym Symbols: TREB5; XBP-1; TREB-5; D11Ertd39e
• NCBI Protein Information: X-box-binding protein 1
• UniProt Protein Name: X-box-binding protein 1
• Protein Family: X-box-binding protein
• UniProt Gene Name: Xbp1
• UniProt Synonym Gene Names: TREB-5Curated

Uniprot Description

Functions as a transcription factor during endoplasmic reticulum stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development and the development of secretory tissues such as exocrine pancreas and salivary gland (PubMed:10425189, PubMed:10652269, PubMed:16362047, PubMed:17612490). Involved in differentiation of B lymphocytes to plasma cells and production of immunoglobulins. Modulates the cellular response to ER stress in a PIK3R-dependent manner. Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes (). Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions (PubMed:23529610). Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention (PubMed:15486293).