Human Methyl CpG Binding Protein 2 (MECP2) ELISA Kit | MECP2 elisa kit

Human Methyl CpG Binding Protein 2 (MECP2) ELISA Kit

Cat. Num. AAA2023499

• Gene Names: MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• Reactivity: Human
• Synonyms: Methyl CpG Binding Protein 2 (MECP2); Human Methyl CpG Binding Protein 2 (MECP2) ELISA Kit; RTS; AUTSX3; MRX16; MRX79; PPMX; RTT; Rett Syndrome; Mental Retardation; X-linked 16; MECP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MECP2. No significant cross-reactivity or interference between MECP2 and analogues was observed.
• Sequence Length: 498

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MECP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MECP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for MECP2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MECP2 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MECP2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MECP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MECP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MECP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MECP2 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 899296
• NCBI GeneID: 4204
• UniProt Accession #: P51608; O15233; Q6QHH9; Q7Z384
• Molecular Weight: 53,323 Da
• NCBI Official Full Name: methyl CpG binding protein 2, partial
• NCBI Official Synonym Full Names: methyl CpG binding protein 2
• NCBI Official Symbol: MECP2
• NCBI Official Synonym Symbols: RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• NCBI Protein Information: methyl-CpG-binding protein 2
• UniProt Protein Name: Methyl-CpG-binding protein 2
• Protein Family: Methyl-CpG-binding protein
• UniProt Gene Name: MECP2
• UniProt Synonym Gene Names: MeCp-2 protein; MeCp2
• UniProt Entry Name: MECP2_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]

Uniprot Description

MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all adult somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; heterochromatin; extracellular space; nucleus; cytosol

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; methyl-CpG binding; DNA binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription corepressor activity; transcription factor binding; transcription factor activity

Biological Process: catecholamine secretion; behavioral fear response; genetic imprinting; startle response; adult locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; pathogenesis; neurological control of breathing; proprioception; negative regulation of transcription from RNA polymerase II promoter; sensory perception of pain; negative regulation of histone methylation; neuron maturation; phosphatidylcholine metabolic process; negative regulation of histone acetylation; post-embryonic development; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; cerebellum development; visual learning; negative regulation of neuron apoptosis; histone acetylation; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; respiratory gaseous exchange; social behavior; cardiolipin metabolic process; histone methylation; positive regulation of synaptogenesis; glucocorticoid metabolic process; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; negative regulation of transcription, DNA-dependent; regulation of excitatory postsynaptic membrane potential

Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Angelman Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations