Mouse Hexosaminidase A Alpha (HEXa) RTU ELISA Kit | HEXa rtu elisa kit

Mouse Hexosaminidase A Alpha (HEXa) ELISA Kit

Cat. Num. AAA459841

• Gene Names: Hexa; Hex-1
• Reactivity: Mouse
• Synonyms: Hexosaminidase A Alpha (HEXa); Mouse Hexosaminidase A Alpha (HEXa) ELISA Kit; TSD; Hex-A; Tay Sachs Disease; GM2 Gangliosidosis; Beta-Hexosaminidase Subunit Alpha; Beta-N-Acetylhexosaminidase Subunit Alpha; N-Acetyl-Beta-Glucosaminidase Alpha; HEXa rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 528

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.051ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HEXa rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HEXa. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HEXa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HEXa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HEXa in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 67514549
• NCBI GeneID: 15211
• NCBI Accession #: NP_034551.2
• NCBI GenBank Nucleotide #: NM_010421.5
• UniProt Accession #: P29416; Q64246; Q91XG3
• Molecular Weight: 60,613 Da
• NCBI Official Full Name: beta-hexosaminidase subunit alpha preproprotein
• NCBI Official Synonym Full Names: hexosaminidase A
• NCBI Official Symbol: Hexa
• NCBI Official Synonym Symbols: Hex-1
• NCBI Protein Information: beta-hexosaminidase subunit alpha
• UniProt Protein Name: Beta-hexosaminidase subunit alpha
• Protein Family: Hexamerin
• UniProt Gene Name: Hexa
• UniProt Synonym Gene Names: Hexosaminidase subunit A

NCBI Description

This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mice lacking the encoded protein exhibit accumulation of gangliosides in the brain and membranous cytoplasmic bodies in neurons. Certain mutations in the human ortholog of this gene cause Tay-Sachs disease. [provided by RefSeq, Aug 2016]

Uniprot Description

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.