Mouse ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) RTU ELISA Kit | ATP1b1 rtu elisa kit

Mouse ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit

Cat. Num. AAA457815

• Gene Names: Atp1b1; Atpb; Atp4b; Atpb-1; NKbeta1
• Reactivity: Mouse
• Synonyms: ATPase; Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1); Mouse ATPase; Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit; ATP1B; Sodium/potassium-dependent ATPase subunit beta-1; ATP1b1 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 304

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.068ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP1b1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP1b1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATP1b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP1b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP1b1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6753138
• NCBI GeneID: 11931
• NCBI Accession #: NP_033851.1
• NCBI GenBank Nucleotide #: NM_009721.6
• UniProt Accession #: P14094
• Molecular Weight: 35,195 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 1 polypeptide
• NCBI Official Symbol: Atp1b1
• NCBI Official Synonym Symbols: Atpb; Atp4b; Atpb-1; NKbeta1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-1
• UniProt Gene Name: Atp1b1
• UniProt Synonym Gene Names: Atp4b

NCBI Description

This gene encodes an integral membrane protein that comprises a subunit of an ATP-metabolizing enzyme responsible for transporting sodium and potassium ions across the plasma membrane. This enzyme regulates the electrochemical gradient of these ions in cells, and plays a central role in osmoregulation and signal transmission in nerves and muscles, among other biological processes. The encoded protein is the non-catalytic beta subunit; it works together with a catalytic alpha subunit and a gamma subunit. [provided by RefSeq, Mar 2013]

Uniprot Description

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.Involved in cell adhesion and establishing epithelial cell polarity.