CD3 epsilon recombinant antibody

Anti-CD3 epsilon [145-2C11]

Cat. Num. AAA488068

• Gene Names: Cd3e; CD3; T3e; AI504783; CD3epsilon
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunoprecipitation
• Purity: Protein A Affinity Purified
• Synonyms: CD3 epsilon; Anti-CD3 epsilon [145-2C11]; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain CD_antigen; CD3e; CD3 epsilon recombinant antibody

• Reactivity: Mouse
• Isotype: Mouse IgG1, kappa
• Clone Number: 145-2C11
• Specificity: Recognizes a 25-kDa protein component (CD3e, originally called T3-e) of the antigen-specific T-cell receptor.
• Purity/Purification: Protein A Affinity Purified
• Sequence Length: 189

• Applicable Applications for CD3 epsilon recombinant antibody: Flow Cytometry (FC/FACS), Western Blot (WB), Immunoprecipitation (IP), Activation
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Immunogen: H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone.
• Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Related Product Information for CD3 epsilon recombinant antibody

Recombinant monoclonal antibody to CD3 epsilon. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 145-2C11. This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Product Categories/Family for CD3 epsilon recombinant antibody

Invivo

References

Leo O, Foo M, Sachs DH, Samelson LE, Bluestone JA. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Natl Acad Sci U S A. 1987 Mar; 84(5):1374-8. PMID:2950524

NCBI and Uniprot Product Information

• NCBI GI #: 1345709
• NCBI GeneID: 12501
• UniProt Accession #: P22646
• Molecular Weight: 21,393 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3 antigen, epsilon polypeptide
• NCBI Official Symbol: Cd3e
• NCBI Official Synonym Symbols: CD3; T3e; AI504783; CD3epsilon
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: Cd3e
• UniProt Entry Name: CD3E_MOUSE

Uniprot Description

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: alpha-beta T cell receptor complex; external side of plasma membrane; immunological synapse; intercellular junction; plasma membrane; T cell receptor complex

Molecular Function: protein binding; protein heterodimerization activity; SH3 domain binding; signal transducer activity

Biological Process: cell surface receptor linked signal transduction; lymphocyte activation; negative regulation of smoothened signaling pathway; negative thymic T cell selection; positive regulation of alpha-beta T cell proliferation; positive regulation of calcium-mediated signaling; positive regulation of interferon-gamma production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell activation; positive regulation of T cell anergy; positive regulation of T cell proliferation; response to nutrient; T cell costimulation; T cell receptor signaling pathway