Rat, Mouse DISC1 Phospho-Regulation Antibody Sampler Kit | DISC1 antibody sampler kit
DISC1 Phospho-Regulation
Reactivity
Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
DISC1 Phospho-Regulation; DISC1 antibody sampler kit
Reactivity
Rat, Mouse
Form/Format
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptides are supplied in PBS and 0.05% sodium azi
Applicable Applications for DISC1 antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C. Stable for 1 year.
Related Product Information for DISC1 antibody sampler kit
Kit Summary: The DISC1 phospho-regulation antibody kit can be used to detect phosphorylation of Ser-710 relative to total DISC1expression. The kit includes two rabbit polyclonal antibodies to detect total expression of DISC1 and secondary reagentsfor detection of rabbit polyclonal antibody in western blot or ELISA. In addition, the kit includes peptides for blockingexperiments or use as ELISA positive controls.
Background: Disrupted in schizophrenia 1 (DISC1) is a multifunctional scaffold protein that has important roles in neurodevelopment.DISC1 is expressed in both neuronal progenitor cells and postmitotic neurons in the developing cerebral cortex. DISC1can interact with both centrosomal proteins and dynein-motor related proteins. In addition, DISC1 interacts with andinhibits the kinase GSK-3beta to enable Wnt activation of beta-catenin-mediated gene transcription and neuron proliferation.These functions of DISC1 may be regulated by post-translational modification. PKA and CDK5 can phosphorylate Ser-710, and a non-phosphorylatable Ser-710 mutant shows decreased interaction with the centrosomal proteins, BBS1 andBBS4, while a constituitively phosphorylated Ser-710 mutant shows increased interaction with these proteins. Duringneuronal progenitor cell proliferation, DISC1 phosphorylation at Ser-710 is low and the interaction with GSK-3beta is enhanced. By contrast, DISC1 phosphorylation is increased, association with GSK-3beta is decreased, and interaction withBBS1 is enhanced during post mitotic neuron migration.
Background: Disrupted in schizophrenia 1 (DISC1) is a multifunctional scaffold protein that has important roles in neurodevelopment.DISC1 is expressed in both neuronal progenitor cells and postmitotic neurons in the developing cerebral cortex. DISC1can interact with both centrosomal proteins and dynein-motor related proteins. In addition, DISC1 interacts with andinhibits the kinase GSK-3beta to enable Wnt activation of beta-catenin-mediated gene transcription and neuron proliferation.These functions of DISC1 may be regulated by post-translational modification. PKA and CDK5 can phosphorylate Ser-710, and a non-phosphorylatable Ser-710 mutant shows decreased interaction with the centrosomal proteins, BBS1 andBBS4, while a constituitively phosphorylated Ser-710 mutant shows increased interaction with these proteins. Duringneuronal progenitor cell proliferation, DISC1 phosphorylation at Ser-710 is low and the interaction with GSK-3beta is enhanced. By contrast, DISC1 phosphorylation is increased, association with GSK-3beta is decreased, and interaction withBBS1 is enhanced during post mitotic neuron migration.
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Product Notes
The Rat, Mouse DISC1 (Catalog #AAA474297) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474297 Antibody Sampler Kit recognizes Rat, Mouse DISC1. AAA Biotech's DISC1 Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the DISC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DISC1 Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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