Interleukin-12/IL-12 Protein | IL-12 protein

Interleukin-12/IL-12

Cat. Num. AAA8533020

• Gene Names: IL12RB1; CD212; IMD30; IL12RB; IL-12R-BETA1
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: Interleukin-12/IL-12; IL-12; Interleukin 12; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; IL-12 protein

• Host: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized
• Sequence Length: 660

• Expression System: Human Cells
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.; It is not recommended to reconstitute to a concentration less than 100 mug/ml.; Dissolve the lyophilized protein in ddH2O.; Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
• Preparation and Storage: Lyophilized protein should be stored at <-20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at <-20 degree C for 3 months.

Related Product Information for IL-12 protein

Background: Mouse IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Human and mouse IL?12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL?12 is active on both human and mouse cells, human IL?12 is not active on murine cells. It is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-gamma. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.
Description: Recombinant Mouse Interleukin-12 is produced by our Mammalian expression system and the target gene encoding Met23-Ser335&Arg23-Ala215 is expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 586946416
• NCBI GeneID: 3594
• NCBI Accession #: NP_001276952.1
• NCBI GenBank Nucleotide #: NM_001290023.1
• UniProt Accession #: P42701; Q8N6Q7; A8K308; B2RPF1; B7ZKK3
• Molecular Weight: 42,366 Da
• NCBI Official Full Name: interleukin-12 receptor subunit beta-1 isoform 3
• NCBI Official Synonym Full Names: interleukin 12 receptor subunit beta 1
• NCBI Official Symbol: IL12RB1
• NCBI Official Synonym Symbols: CD212; IMD30; IL12RB; IL-12R-BETA1
• NCBI Protein Information: interleukin-12 receptor subunit beta-1
• UniProt Protein Name: Interleukin-12 receptor subunit beta-1
• UniProt Gene Name: IL12RB1
• UniProt Synonym Gene Names: IL12R; IL12RB; IL-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12R-beta-1; IL-12RB1

NCBI Description

The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.